Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T

Background: Human T-lymphotropic virus-1 (HTLV-1) infection has been associated with the pathogenesis of cutaneous T cell lymphomas (CTCL). Aim: To search for HTLV-1 DNA in skin biopsies of patients with CTCL. Material and Methods: A retrospective study was conducted using 25 biopsies of patients wi...

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Autores principales:	Benedetto E,Juana, Molgó N,Montserrat, González B,Sergio
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2014
Materias:	Human T-lymphotropic virus 1 Lymphoma T-cell cutaneous Polymerase chain reaction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872014000300005
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spelling	oai:scielo:S0034-988720140003000052014-09-02Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células TBenedetto E,JuanaMolgó N,MontserratGonzález B,Sergio Human T-lymphotropic virus 1 Lymphoma T-cell cutaneous Polymerase chain reaction Background: Human T-lymphotropic virus-1 (HTLV-1) infection has been associated with the pathogenesis of cutaneous T cell lymphomas (CTCL). Aim: To search for HTLV-1 DNA in skin biopsies of patients with CTCL. Material and Methods: A retrospective study was conducted using 25 biopsies of patients with CTCL. DNA was extracted from lymphoid tissue by microdissection. A nested PCR was conducted to detect HTLV-1 genome using primers for the tax region. As negative controls, four cases of superficial perivascular dermatitis were chosen. As positive controls, five cases of T-cell leukemia/lymphoma (ATCL) were studied. Results: A positive reaction was found in 3 of 25 cases. These biopsies corresponded to a case of Mycosis Fungoides, a case of CD30 (-) T-cell lymphoma and a case of lymphomatoid papulosis. Search was negative in the four cases of superficial perivascular dermatitis and positive in four cases of adult T-cell leukemia/lymphoma (ATCL). Conclusions: HTLV-1 DNA search in tissues is a useful tool recommended to study T-cell lymphomas. HTLV-1 infection only occurs in sporadic cases but may contribute to tumor aggressiveness and prognosis.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.142 n.3 20142014-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872014000300005es10.4067/S0034-98872014000300005
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Human T-lymphotropic virus 1 Lymphoma T-cell cutaneous Polymerase chain reaction
spellingShingle	Human T-lymphotropic virus 1 Lymphoma T-cell cutaneous Polymerase chain reaction Benedetto E,Juana Molgó N,Montserrat González B,Sergio Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T
description	Background: Human T-lymphotropic virus-1 (HTLV-1) infection has been associated with the pathogenesis of cutaneous T cell lymphomas (CTCL). Aim: To search for HTLV-1 DNA in skin biopsies of patients with CTCL. Material and Methods: A retrospective study was conducted using 25 biopsies of patients with CTCL. DNA was extracted from lymphoid tissue by microdissection. A nested PCR was conducted to detect HTLV-1 genome using primers for the tax region. As negative controls, four cases of superficial perivascular dermatitis were chosen. As positive controls, five cases of T-cell leukemia/lymphoma (ATCL) were studied. Results: A positive reaction was found in 3 of 25 cases. These biopsies corresponded to a case of Mycosis Fungoides, a case of CD30 (-) T-cell lymphoma and a case of lymphomatoid papulosis. Search was negative in the four cases of superficial perivascular dermatitis and positive in four cases of adult T-cell leukemia/lymphoma (ATCL). Conclusions: HTLV-1 DNA search in tissues is a useful tool recommended to study T-cell lymphomas. HTLV-1 infection only occurs in sporadic cases but may contribute to tumor aggressiveness and prognosis.
author	Benedetto E,Juana Molgó N,Montserrat González B,Sergio
author_facet	Benedetto E,Juana Molgó N,Montserrat González B,Sergio
author_sort	Benedetto E,Juana
title	Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T
title_short	Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T
title_full	Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T
title_fullStr	Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T
title_full_unstemmed	Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T
title_sort	búsqueda del adn del virus htlv-1 en biopsias de pacientes con linfoma cutáneo de células t
publisher	Sociedad Médica de Santiago
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872014000300005
work_keys_str_mv	AT benedettoejuana busquedadeladndelvirushtlv1enbiopsiasdepacientesconlinfomacutaneodecelulast AT molgonmontserrat busquedadeladndelvirushtlv1enbiopsiasdepacientesconlinfomacutaneodecelulast AT gonzalezbsergio busquedadeladndelvirushtlv1enbiopsiasdepacientesconlinfomacutaneodecelulast
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Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T

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