Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

Background: Ozone exposure could increase lung damage induced by airborne particulate matter. Particulate matter lung toxicity has been attributed to its metallic content. Aim: To evaluate the acute effect of intratracheal administration of copper sulfate (CuSO4) on rat lungs previously damaged by a...

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Autores principales:	Oyarzún G,Manuel J, Sánchez R,Susan A, Dussaubat D,Nelson, Miller A,María E, González B,Sergio
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2017
Materias:	F2-Isoprostanes Lung Injury Macrophages, Alveolar Ozone Particulate Matter
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872017000100002
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spelling	oai:scielo:S0034-988720170001000022017-05-15Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozonoOyarzún G,Manuel JSánchez R,Susan ADussaubat D,NelsonMiller A,María EGonzález B,Sergio F2-Isoprostanes Lung Injury Macrophages, Alveolar Ozone Particulate Matter Background: Ozone exposure could increase lung damage induced by airborne particulate matter. Particulate matter lung toxicity has been attributed to its metallic content. Aim: To evaluate the acute effect of intratracheal administration of copper sulfate (CuSO4) on rat lungs previously damaged by a chronic intermittent ozone exposure. Material and Methods: Two-months-old male Sprague-Dawley rats were exposed to 0.5 ppm ozone four h per day, five days a week, during two months. CuSO4 was intratracheally instilled 20 h after ozone exposure. Controls breathed filtered air or were instilled with 0.9% NaCl or with CuSO4 or were only exposed to ozone. We evaluated lung histopathology. F2 isoprostanes were determined in plasma. Cell count, total proteins, γ glutamyl-transpeptidase (GGT) and alkaline phosphatases (AP) were determined in bronchoalveolar lavage fluid (BALF). Results: Ozone increased total cell count, macrophages, proteins and AP in BALF (p < 0.05), and induced pulmonary neutrophil inflammation. CuSO4 plus air increased plasma F2 isoprostane levels and total cell count, neutrophils and proteins in BALF (p < 0.05). Histopathology showed foamy macrophages. Ozone plus CuSO4 exposed animals showed a neutrophil inflammatory lung response and an increase in total cell count, proteins, GGT and AP in BALF (p < 0.05). Foamy and pigmented alveolar macrophages were detected in all lungs of these animals (p < 0.001). Conclusions: Intratracheal instillation of a single dose of CuSO4 in rats previously subjected to a chronic and intermittent exposure to ozone induces a neutrophil pulmonary inflammatory response and cytoplasmic damage in macrophages.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.145 n.1 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872017000100002es10.4067/S0034-98872017000100002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	F2-Isoprostanes Lung Injury Macrophages, Alveolar Ozone Particulate Matter
spellingShingle	F2-Isoprostanes Lung Injury Macrophages, Alveolar Ozone Particulate Matter Oyarzún G,Manuel J Sánchez R,Susan A Dussaubat D,Nelson Miller A,María E González B,Sergio Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
description	Background: Ozone exposure could increase lung damage induced by airborne particulate matter. Particulate matter lung toxicity has been attributed to its metallic content. Aim: To evaluate the acute effect of intratracheal administration of copper sulfate (CuSO4) on rat lungs previously damaged by a chronic intermittent ozone exposure. Material and Methods: Two-months-old male Sprague-Dawley rats were exposed to 0.5 ppm ozone four h per day, five days a week, during two months. CuSO4 was intratracheally instilled 20 h after ozone exposure. Controls breathed filtered air or were instilled with 0.9% NaCl or with CuSO4 or were only exposed to ozone. We evaluated lung histopathology. F2 isoprostanes were determined in plasma. Cell count, total proteins, γ glutamyl-transpeptidase (GGT) and alkaline phosphatases (AP) were determined in bronchoalveolar lavage fluid (BALF). Results: Ozone increased total cell count, macrophages, proteins and AP in BALF (p < 0.05), and induced pulmonary neutrophil inflammation. CuSO4 plus air increased plasma F2 isoprostane levels and total cell count, neutrophils and proteins in BALF (p < 0.05). Histopathology showed foamy macrophages. Ozone plus CuSO4 exposed animals showed a neutrophil inflammatory lung response and an increase in total cell count, proteins, GGT and AP in BALF (p < 0.05). Foamy and pigmented alveolar macrophages were detected in all lungs of these animals (p < 0.001). Conclusions: Intratracheal instillation of a single dose of CuSO4 in rats previously subjected to a chronic and intermittent exposure to ozone induces a neutrophil pulmonary inflammatory response and cytoplasmic damage in macrophages.
author	Oyarzún G,Manuel J Sánchez R,Susan A Dussaubat D,Nelson Miller A,María E González B,Sergio
author_facet	Oyarzún G,Manuel J Sánchez R,Susan A Dussaubat D,Nelson Miller A,María E González B,Sergio
author_sort	Oyarzún G,Manuel J
title	Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
title_short	Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
title_full	Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
title_fullStr	Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
title_full_unstemmed	Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
title_sort	efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono
publisher	Sociedad Médica de Santiago
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872017000100002
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Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

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