Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol

Background: It is known that some nutrients play an important role in the development of cholelithiasis. Cholesterol is carried by micelles and vesicles in the bile. During the first stage of gallstone formation, cholesterol crystals derive from thermodynamically unstable vesicles. Aim: To determi...

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Autores principales:	Del Pozo,Reginald, Mardones,Lorena, Villagrán,Marcelo, Muñoz,Katia, Roa,Susana, Rozas,Francisca, Ormazábal,Valeska, Muñoz,Mirna
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2017
Materias:	Cholelithiasis Dietary Fats Lipid Metabolism
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872017000901099
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spelling	oai:scielo:S0034-988720170009010992017-12-07Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterolDel Pozo,ReginaldMardones,LorenaVillagrán,MarceloMuñoz,KatiaRoa,SusanaRozas,FranciscaOrmazábal,ValeskaMuñoz,Mirna Cholelithiasis Dietary Fats Lipid Metabolism Background: It is known that some nutrients play an important role in the development of cholelithiasis. Cholesterol is carried by micelles and vesicles in the bile. During the first stage of gallstone formation, cholesterol crystals derive from thermodynamically unstable vesicles. Aim: To determine the effect of a high fat diet on blood lipids and bile composition, and its implication in the formation of gallstones. Material and Methods: Two groups of 15 BALB/c mice each, coming from the same litter, were treated with a control or with a high-fat diet (64% fat and 0.14% cholesterol). After two months, the animals were sacrificed, blood and bile samples were obtained. Serum glucose and the corresponding lipid profiles were measured. In bile samples, cholesterol and phospholipid levels were analyzed, and cholesterol transporters (vesicles and micelles) were separated by gel filtration chromatography. Results: Treated animals showed an 87% increase in serum total cholesterol (p < 0.01), a 97% increase in HDL-cholesterol (p < 0.05) and a 140% increase in LDL-cholesterol (p < 0.05). No changes in serum triglycerides or glucose were observed. In bile, a 13% increase in biliary cholesterol (p < 0.05) was observed but no change in biliary phospholipids. Also, an increase in biliary vesicular transporters and an increase of cholesterol/phospholipid ratio in vesicular transporters were observed. Conclusions: A high fat diet may contribute to the formation of gallstones in our experimental model.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.145 n.9 20172017-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872017000901099es10.4067/s0034-98872017000901099
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Cholelithiasis Dietary Fats Lipid Metabolism
spellingShingle	Cholelithiasis Dietary Fats Lipid Metabolism Del Pozo,Reginald Mardones,Lorena Villagrán,Marcelo Muñoz,Katia Roa,Susana Rozas,Francisca Ormazábal,Valeska Muñoz,Mirna Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
description	Background: It is known that some nutrients play an important role in the development of cholelithiasis. Cholesterol is carried by micelles and vesicles in the bile. During the first stage of gallstone formation, cholesterol crystals derive from thermodynamically unstable vesicles. Aim: To determine the effect of a high fat diet on blood lipids and bile composition, and its implication in the formation of gallstones. Material and Methods: Two groups of 15 BALB/c mice each, coming from the same litter, were treated with a control or with a high-fat diet (64% fat and 0.14% cholesterol). After two months, the animals were sacrificed, blood and bile samples were obtained. Serum glucose and the corresponding lipid profiles were measured. In bile samples, cholesterol and phospholipid levels were analyzed, and cholesterol transporters (vesicles and micelles) were separated by gel filtration chromatography. Results: Treated animals showed an 87% increase in serum total cholesterol (p < 0.01), a 97% increase in HDL-cholesterol (p < 0.05) and a 140% increase in LDL-cholesterol (p < 0.05). No changes in serum triglycerides or glucose were observed. In bile, a 13% increase in biliary cholesterol (p < 0.05) was observed but no change in biliary phospholipids. Also, an increase in biliary vesicular transporters and an increase of cholesterol/phospholipid ratio in vesicular transporters were observed. Conclusions: A high fat diet may contribute to the formation of gallstones in our experimental model.
author	Del Pozo,Reginald Mardones,Lorena Villagrán,Marcelo Muñoz,Katia Roa,Susana Rozas,Francisca Ormazábal,Valeska Muñoz,Mirna
author_facet	Del Pozo,Reginald Mardones,Lorena Villagrán,Marcelo Muñoz,Katia Roa,Susana Rozas,Francisca Ormazábal,Valeska Muñoz,Mirna
author_sort	Del Pozo,Reginald
title	Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
title_short	Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
title_full	Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
title_fullStr	Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
title_full_unstemmed	Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
title_sort	efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol
publisher	Sociedad Médica de Santiago
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872017000901099
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Efecto de una dieta alta en grasas en el proceso de formación de cálculos biliares de colesterol

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