Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales

Background: Helicobacter pylori is the most significant pathogen associated with gastric diseases, including gastric cancer. Infected patients with strains that are CagA-positive generally have worse outcomes than those infected with CagA-negative strains. Patients infected with CagA-positive strai...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wormwood,Tracy, Parra,Álvaro, Bresky,Gustavo, Madariaga,Juan A., Häberle,Sergio, Flores,Jacqueline, Bernal,Giuliano
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2018
Materias:	Bacterial infections Feces Helicobacter pylori Molecular Epidemiology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872018000500596
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0034-98872018000500596
record_format	dspace
spelling	oai:scielo:S0034-988720180005005962018-07-26Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecalesWormwood,TracyParra,ÁlvaroBresky,GustavoMadariaga,Juan A.Häberle,SergioFlores,JacquelineBernal,Giuliano Bacterial infections Feces Helicobacter pylori Molecular Epidemiology Background: Helicobacter pylori is the most significant pathogen associated with gastric diseases, including gastric cancer. Infected patients with strains that are CagA-positive generally have worse outcomes than those infected with CagA-negative strains. Patients infected with CagA-positive strains have a higher risk for developing gastric cancer. Aim: To determine the prevalence of CagA-positive H. pylori strains in fecal samples of patients from the Coquimbo Region of Chile, using a non-invasive, nested-qPCR method. Material and Methods: We evaluated 160 patients with gastrointestinal symptoms subjected to an upper gastrointestinal endoscopy. DNA was extracted from fecal samples and tested for the presence of H. pylori using nested-qPCR for the ureC gene, and subsequently compared with the results of histology-Giemsa stain from the patients' endoscopic biopsies. When H. pylori was found, the presence of CagA-positive strains was determined via nested-qPCR. Results: The histology-Giemsa stain was positive for H. pylori infection in 123 patients (76.9%), while the analysis of fecal samples detected H. pylori in 129 patients (80.6%). The sensitivity and specificity of nested-qPCR to detect the bacterium was 96.7 and 73.0% respectively. Among patients with the infection, 25% had CagA-positive strains. Conclusions: In this sample of patients, there is a low prevalence of CagA-positive H. pylori strains.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.146 n.5 20182018-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872018000500596es10.4067/s0034-98872018000500596
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Bacterial infections Feces Helicobacter pylori Molecular Epidemiology
spellingShingle	Bacterial infections Feces Helicobacter pylori Molecular Epidemiology Wormwood,Tracy Parra,Álvaro Bresky,Gustavo Madariaga,Juan A. Häberle,Sergio Flores,Jacqueline Bernal,Giuliano Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales
description	Background: Helicobacter pylori is the most significant pathogen associated with gastric diseases, including gastric cancer. Infected patients with strains that are CagA-positive generally have worse outcomes than those infected with CagA-negative strains. Patients infected with CagA-positive strains have a higher risk for developing gastric cancer. Aim: To determine the prevalence of CagA-positive H. pylori strains in fecal samples of patients from the Coquimbo Region of Chile, using a non-invasive, nested-qPCR method. Material and Methods: We evaluated 160 patients with gastrointestinal symptoms subjected to an upper gastrointestinal endoscopy. DNA was extracted from fecal samples and tested for the presence of H. pylori using nested-qPCR for the ureC gene, and subsequently compared with the results of histology-Giemsa stain from the patients' endoscopic biopsies. When H. pylori was found, the presence of CagA-positive strains was determined via nested-qPCR. Results: The histology-Giemsa stain was positive for H. pylori infection in 123 patients (76.9%), while the analysis of fecal samples detected H. pylori in 129 patients (80.6%). The sensitivity and specificity of nested-qPCR to detect the bacterium was 96.7 and 73.0% respectively. Among patients with the infection, 25% had CagA-positive strains. Conclusions: In this sample of patients, there is a low prevalence of CagA-positive H. pylori strains.
author	Wormwood,Tracy Parra,Álvaro Bresky,Gustavo Madariaga,Juan A. Häberle,Sergio Flores,Jacqueline Bernal,Giuliano
author_facet	Wormwood,Tracy Parra,Álvaro Bresky,Gustavo Madariaga,Juan A. Häberle,Sergio Flores,Jacqueline Bernal,Giuliano
author_sort	Wormwood,Tracy
title	Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales
title_short	Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales
title_full	Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales
title_fullStr	Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales
title_full_unstemmed	Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales
title_sort	prevalencia de cepas caga-positivo en la región de coquimbo, determinada mediante nested-qpcr en muestras fecales
publisher	Sociedad Médica de Santiago
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872018000500596
work_keys_str_mv	AT wormwoodtracy prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales AT parraalvaro prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales AT breskygustavo prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales AT madariagajuana prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales AT haberlesergio prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales AT floresjacqueline prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales AT bernalgiuliano prevalenciadecepascagapositivoenlaregiondecoquimbodeterminadamediantenestedqpcrenmuestrasfecales
_version_	1718437005306626048

Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales

Ejemplares similares