Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno

Caseins are a family of milk proteins that exist in several molecular forms and are the main proteins present in the bovine milk. Genetic variants of these proteins have been associated with the quality and quantity of cheese derived from milk. Genotypes of 278 Frisón Negro Chileno cows were determi...

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Autores principales:	FELMER,R., BUTENDIECK,N.
Lenguaje:	Spanish / Castilian
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 1998
Materias:	<FONT FACE=Symbol>k</FONT>-caseína PCR genotipo
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1998000200015
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spelling	oai:scielo:S0301-732X19980002000152000-09-12Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro ChilenoFELMER,R.BUTENDIECK,N. <FONT FACE=Symbol>k</FONT>-caseína PCR genotipo Caseins are a family of milk proteins that exist in several molecular forms and are the main proteins present in the bovine milk. Genetic variants of these proteins have been associated with the quality and quantity of cheese derived from milk. Genotypes of 278 Frisón Negro Chileno cows were determined for <FONT FACE=Symbol>k</FONT>-casein by restriction fragment length polymorphism analysis of amplified DNA. A 350 bp fragment of the genomic bovine <FONT FACE=Symbol>k</FONT>-casein gene was amplified by PCR. Two HINF I sites were found in the amplified fragment of allele A, one at position 134 and one at position 266; only the latter site is present in allele B. Thus, digests of alleles A yielded 84 bp and 132/134 bp bands and digests of alleles B resulted in 84 bp and 266 bp bands. These bands, and thus genotypes AA, AB and BB, were recognised by agarose gel electrophoresis and ethidium-bromide staining. This technique was used to determine the <FONT FACE=Symbol>k</FONT>-casein allelic frequency in a Frisón Negro dairy herd. The distribution of genotypes was slightly different, and the gene frequencies similar to those reported in the literature. This molecular genetic technique based on molecular markers allows direct genotyping for milk <FONT FACE=Symbol>k</FONT>-casein with certainty and accuracy in bulls and females to be used in programs of dairy cattle improvement. Therefore, an early and precise identification of milk protein genotypes should have a direct impact on dairy cattle breeding strategiesinfo:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.30 n.2 19981998-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1998000200015es10.4067/S0301-732X1998000200015
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	<FONT FACE=Symbol>k</FONT>-caseína PCR genotipo
spellingShingle	<FONT FACE=Symbol>k</FONT>-caseína PCR genotipo FELMER,R. BUTENDIECK,N. Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno
description	Caseins are a family of milk proteins that exist in several molecular forms and are the main proteins present in the bovine milk. Genetic variants of these proteins have been associated with the quality and quantity of cheese derived from milk. Genotypes of 278 Frisón Negro Chileno cows were determined for <FONT FACE=Symbol>k</FONT>-casein by restriction fragment length polymorphism analysis of amplified DNA. A 350 bp fragment of the genomic bovine <FONT FACE=Symbol>k</FONT>-casein gene was amplified by PCR. Two HINF I sites were found in the amplified fragment of allele A, one at position 134 and one at position 266; only the latter site is present in allele B. Thus, digests of alleles A yielded 84 bp and 132/134 bp bands and digests of alleles B resulted in 84 bp and 266 bp bands. These bands, and thus genotypes AA, AB and BB, were recognised by agarose gel electrophoresis and ethidium-bromide staining. This technique was used to determine the <FONT FACE=Symbol>k</FONT>-casein allelic frequency in a Frisón Negro dairy herd. The distribution of genotypes was slightly different, and the gene frequencies similar to those reported in the literature. This molecular genetic technique based on molecular markers allows direct genotyping for milk <FONT FACE=Symbol>k</FONT>-casein with certainty and accuracy in bulls and females to be used in programs of dairy cattle improvement. Therefore, an early and precise identification of milk protein genotypes should have a direct impact on dairy cattle breeding strategies
author	FELMER,R. BUTENDIECK,N.
author_facet	FELMER,R. BUTENDIECK,N.
author_sort	FELMER,R.
title	Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno
title_short	Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno
title_full	Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno
title_fullStr	Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno
title_full_unstemmed	Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno
title_sort	frecuencia alélica del gen de la k-caseína bovina en un rebaño frisón negro chileno
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	1998
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1998000200015
work_keys_str_mv	AT felmerr frecuenciaalelicadelgendelakcaseinabovinaenunrebanofrisonnegrochileno AT butendieckn frecuenciaalelicadelgendelakcaseinabovinaenunrebanofrisonnegrochileno
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Frecuencia alélica del gen de la k-caseína bovina en un rebaño Frisón Negro Chileno

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