A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats
A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon ant...
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| Lenguaje: | English |
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Facultad de Ciencias Veterinarias, Universidad Austral de Chile
1999
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oai:scielo:S0301-732X19990001000152000-08-08A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goatsUZAL,F.A.SMITH,P.NIELSEN,K.LIN,M.KELLY,W.R. fluorescence polarization assay goats Clostridium perfringens epsilon toxin antibodies A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the laterinfo:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.31 n.1 19991999-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1999000100015en10.4067/S0301-732X1999000100015 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
fluorescence polarization assay goats Clostridium perfringens epsilon toxin antibodies |
| spellingShingle |
fluorescence polarization assay goats Clostridium perfringens epsilon toxin antibodies UZAL,F.A. SMITH,P. NIELSEN,K. LIN,M. KELLY,W.R. A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats |
| description |
A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later |
| author |
UZAL,F.A. SMITH,P. NIELSEN,K. LIN,M. KELLY,W.R. |
| author_facet |
UZAL,F.A. SMITH,P. NIELSEN,K. LIN,M. KELLY,W.R. |
| author_sort |
UZAL,F.A. |
| title |
A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats |
| title_short |
A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats |
| title_full |
A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats |
| title_fullStr |
A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats |
| title_full_unstemmed |
A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats |
| title_sort |
homogeneous fluorescence polarization assay for detection of clostridium perfringenstype d epsilon antitoxin in serum of goats |
| publisher |
Facultad de Ciencias Veterinarias, Universidad Austral de Chile |
| publishDate |
1999 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1999000100015 |
| work_keys_str_mv |
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| _version_ |
1718437785391595520 |