Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1
The aim of this study was to determine the consequences of the infection of BVDV genotype II (BVDV-2) in calves with preexisting immunity to BVDV-1 (induced by infection with wild type BVDV (Group A, 4 calves), or induced by vaccination with a killed BVDV-1 product (Group B, 2 calves). Group A and B...
Guardado en:
Autores principales: | , , , |
---|---|
Lenguaje: | Spanish / Castilian |
Publicado: |
Facultad de Ciencias Veterinarias, Universidad Austral de Chile
2001
|
Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2001000200007 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:scielo:S0301-732X2001000200007 |
---|---|
record_format |
dspace |
spelling |
oai:scielo:S0301-732X20010002000072005-09-20Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1Ronchi,J.I.Estela,E.SLeunda,M. ROdeón,A. C Virus de la Diarrea Viral Bovina VDVB infección experimental genotipos inmunidad vacuna inactivada The aim of this study was to determine the consequences of the infection of BVDV genotype II (BVDV-2) in calves with preexisting immunity to BVDV-1 (induced by infection with wild type BVDV (Group A, 4 calves), or induced by vaccination with a killed BVDV-1 product (Group B, 2 calves). Group A and B were intranasally exposed to a field isolate of BVDV-2 (25ml x 10-7.8 TCID50/ml). Calves of Group A had erosions on the oral mucosa from 3 to 4 day post-challenge (DPC); moreover, intense salivation, mucous nasal secretion and sporadic diarrhea were observed from 4-14 DPC. Only one calf of Group B had diarrhea at 5 DPC. The average value of peripheral blood leukocytes (PBL) previous to BVDV-2 challenge was 8,775 PBL/ml (±603) in calves of Group A and 9,975 PBL/ml (±1308) in calves of Group B. After BVDV-2 challenge there was leukopenia in Groups A and B. The minimal number of PBL of 5,875 PBL/ml (±311) was observed in Group A at 6 PDC (P <0.05). There were differences (P <0,05) in the average of PBL during the 17 DPC among challenged animals and uninfected controls. Moreover, a difference in the number of blood lymphocytes was observed at 6-7 DPC between virus exposed and control calves. BVDV was isolated from ocular swabs of 3 calves of Group A at 13 and 17 DPC and from PBL at 9 and 11 DPC. The neutralizing antibody titer (NT) to both genotypes of BVDV was higher (P <0.05) in calves of Group A. Previous to virus challenge calves in this group had NT of 1:125 (geometric mean) and 1:128 to BVDV-1 and -II, respectively. The NT rose to 1:2048 at 17 DPC to both Genotypes of BVDV. Calves in Group B had a NT of 1:16 to both BVDV-1 and -II previous to BVDV-2 challenge. At 17 DPC the NT rose to 1:64 and to 1:181 to BVDV-1 and -II, respectively. These results allow us to conclude that antibodies generated by natural infection or vaccination with BVDV-1 increased for BVDV-1 and -2 after the experimental challenge with BVDV-2.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.33 n.2 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2001000200007es10.4067/S0301-732X2001000200007 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
Spanish / Castilian |
topic |
Virus de la Diarrea Viral Bovina VDVB infección experimental genotipos inmunidad vacuna inactivada |
spellingShingle |
Virus de la Diarrea Viral Bovina VDVB infección experimental genotipos inmunidad vacuna inactivada Ronchi,J.I. Estela,E.S Leunda,M. R Odeón,A. C Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 |
description |
The aim of this study was to determine the consequences of the infection of BVDV genotype II (BVDV-2) in calves with preexisting immunity to BVDV-1 (induced by infection with wild type BVDV (Group A, 4 calves), or induced by vaccination with a killed BVDV-1 product (Group B, 2 calves). Group A and B were intranasally exposed to a field isolate of BVDV-2 (25ml x 10-7.8 TCID50/ml). Calves of Group A had erosions on the oral mucosa from 3 to 4 day post-challenge (DPC); moreover, intense salivation, mucous nasal secretion and sporadic diarrhea were observed from 4-14 DPC. Only one calf of Group B had diarrhea at 5 DPC. The average value of peripheral blood leukocytes (PBL) previous to BVDV-2 challenge was 8,775 PBL/ml (±603) in calves of Group A and 9,975 PBL/ml (±1308) in calves of Group B. After BVDV-2 challenge there was leukopenia in Groups A and B. The minimal number of PBL of 5,875 PBL/ml (±311) was observed in Group A at 6 PDC (P <0.05). There were differences (P <0,05) in the average of PBL during the 17 DPC among challenged animals and uninfected controls. Moreover, a difference in the number of blood lymphocytes was observed at 6-7 DPC between virus exposed and control calves. BVDV was isolated from ocular swabs of 3 calves of Group A at 13 and 17 DPC and from PBL at 9 and 11 DPC. The neutralizing antibody titer (NT) to both genotypes of BVDV was higher (P <0.05) in calves of Group A. Previous to virus challenge calves in this group had NT of 1:125 (geometric mean) and 1:128 to BVDV-1 and -II, respectively. The NT rose to 1:2048 at 17 DPC to both Genotypes of BVDV. Calves in Group B had a NT of 1:16 to both BVDV-1 and -II previous to BVDV-2 challenge. At 17 DPC the NT rose to 1:64 and to 1:181 to BVDV-1 and -II, respectively. These results allow us to conclude that antibodies generated by natural infection or vaccination with BVDV-1 increased for BVDV-1 and -2 after the experimental challenge with BVDV-2. |
author |
Ronchi,J.I. Estela,E.S Leunda,M. R Odeón,A. C |
author_facet |
Ronchi,J.I. Estela,E.S Leunda,M. R Odeón,A. C |
author_sort |
Ronchi,J.I. |
title |
Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 |
title_short |
Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 |
title_full |
Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 |
title_fullStr |
Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 |
title_full_unstemmed |
Infección experimental con el virus de la diarrea viral bovina (VDVB) genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 |
title_sort |
infección experimental con el virus de la diarrea viral bovina (vdvb) genotipo 2 en terneros con anticuerpos neutralizantes al vdvb genotipo 1 |
publisher |
Facultad de Ciencias Veterinarias, Universidad Austral de Chile |
publishDate |
2001 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2001000200007 |
work_keys_str_mv |
AT ronchiji infeccionexperimentalconelvirusdeladiarreaviralbovinavdvbgenotipo2enternerosconanticuerposneutralizantesalvdvbgenotipo1 AT estelaes infeccionexperimentalconelvirusdeladiarreaviralbovinavdvbgenotipo2enternerosconanticuerposneutralizantesalvdvbgenotipo1 AT leundamr infeccionexperimentalconelvirusdeladiarreaviralbovinavdvbgenotipo2enternerosconanticuerposneutralizantesalvdvbgenotipo1 AT odeonac infeccionexperimentalconelvirusdeladiarreaviralbovinavdvbgenotipo2enternerosconanticuerposneutralizantesalvdvbgenotipo1 |
_version_ |
1718437804536496128 |