Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)

Growing international competition is forcing salmon farmers to incorporate innovative techniques into the production process. The use of triploid, all-female breeding populations offers multiple advantages over diploid populations. Currently, an exact, simple, and non- hazardous method for the quant...

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Autores principales:	Härtel,S, Rojas,R, Räth,C, Guarda,M I, Goicoechea,O
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2005
Materias:	triploidization cytometry microscopy image processing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2005000200009
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spelling	oai:scielo:S0301-732X20050002000092006-01-10Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)Härtel,SRojas,RRäth,CGuarda,M IGoicoechea,O triploidization cytometry microscopy image processing Growing international competition is forcing salmon farmers to incorporate innovative techniques into the production process. The use of triploid, all-female breeding populations offers multiple advantages over diploid populations. Currently, an exact, simple, and non- hazardous method for the quantification of diploid- and triploid salmon erythrocytes does not exist. We present a method that combines a standard microscopic bright field technique (contrast staining with GIEMSA) with multi-parameter image analysis and termed it quantitative morphologic microscopy (QMM). We used flow cytometry (FC) as the reference method to determine the DNA content of di- and triplod erythrocytes from immature rainbow trout (Oncorhynchus mykiss). Additionally, we applied quantitative fluorescence microscopy (QFM), using the DNA stains 4',6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), and acridine orange (AO). Our data show that QMM possess comparable or even superior discriminating capacities than FC or QFM. The developed method opens a perspective for the classification of microscopic objects with many possible applicationsinfo:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.37 n.2 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2005000200009en10.4067/S0301-732X2005000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	triploidization cytometry microscopy image processing
spellingShingle	triploidization cytometry microscopy image processing Härtel,S Rojas,R Räth,C Guarda,M I Goicoechea,O Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)
description	Growing international competition is forcing salmon farmers to incorporate innovative techniques into the production process. The use of triploid, all-female breeding populations offers multiple advantages over diploid populations. Currently, an exact, simple, and non- hazardous method for the quantification of diploid- and triploid salmon erythrocytes does not exist. We present a method that combines a standard microscopic bright field technique (contrast staining with GIEMSA) with multi-parameter image analysis and termed it quantitative morphologic microscopy (QMM). We used flow cytometry (FC) as the reference method to determine the DNA content of di- and triplod erythrocytes from immature rainbow trout (Oncorhynchus mykiss). Additionally, we applied quantitative fluorescence microscopy (QFM), using the DNA stains 4',6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), and acridine orange (AO). Our data show that QMM possess comparable or even superior discriminating capacities than FC or QFM. The developed method opens a perspective for the classification of microscopic objects with many possible applications
author	Härtel,S Rojas,R Räth,C Guarda,M I Goicoechea,O
author_facet	Härtel,S Rojas,R Räth,C Guarda,M I Goicoechea,O
author_sort	Härtel,S
title	Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)
title_short	Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)
title_full	Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)
title_fullStr	Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)
title_full_unstemmed	Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)
title_sort	di- and triploid erythrocyte identification by multi-parameter image analysis: a new method for the quantification of triploidization rates in rainbow trout (oncorhynchus mykiss)
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2005000200009
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Di- and triploid erythrocyte identification by multi-parameter image analysis: A new method for the quantification of triploidization rates in rainbow trout (Oncorhynchus mykiss)

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