Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay

Laboratory diagnosis of brucellosis is generally performed by microbiological and serological methods. PCR assay is a specific and sensitive choice for the detection of different bacterial agents. An evaluation of this test was carried out for the detection of Brucella melitensis DNA in sheep milk....

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Autores principales:	Ilhan,Z, Solmaz,H, Aksakal,A, Gulhan,T, Ekin,IH, Boynukara,B
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2008
Materias:	Brucella melitensis sheep milk PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2008000200005
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spelling	oai:scielo:S0301-732X20080002000052009-01-27Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assayIlhan,ZSolmaz,HAksakal,AGulhan,TEkin,IHBoynukara,B Brucella melitensis sheep milk PCR Laboratory diagnosis of brucellosis is generally performed by microbiological and serological methods. PCR assay is a specific and sensitive choice for the detection of different bacterial agents. An evaluation of this test was carried out for the detection of Brucella melitensis DNA in sheep milk. 102 milk samples from sheep after abortion were taken and studied using bacteriological culture, PCR and milk ring test (MRT). PCR found B. melitensis DNA in 24 (23.5%) out of 102 milk samples, while only 8 (7.8%) of the samples were positive to B. melitensis through direct culture. MRT found 28 (27.4%) positive milk samples. The detection limit for PCR in sheep milk inoculated with B. melitensis strain 16 M was 1.7x10³-1.7x10(4) cfu/ml. PCR and MRT coincidence was 96%. The diagnostic sensitivity and specificity were determined as 100% and 81.3% respectively for PCR assay and 75% and 75% for MRT. PCR is a useful tool for a fast diagnosis of B. melitensis in sheep milk.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.40 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2008000200005en10.4067/S0301-732X2008000200005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Brucella melitensis sheep milk PCR
spellingShingle	Brucella melitensis sheep milk PCR Ilhan,Z Solmaz,H Aksakal,A Gulhan,T Ekin,IH Boynukara,B Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay
description	Laboratory diagnosis of brucellosis is generally performed by microbiological and serological methods. PCR assay is a specific and sensitive choice for the detection of different bacterial agents. An evaluation of this test was carried out for the detection of Brucella melitensis DNA in sheep milk. 102 milk samples from sheep after abortion were taken and studied using bacteriological culture, PCR and milk ring test (MRT). PCR found B. melitensis DNA in 24 (23.5%) out of 102 milk samples, while only 8 (7.8%) of the samples were positive to B. melitensis through direct culture. MRT found 28 (27.4%) positive milk samples. The detection limit for PCR in sheep milk inoculated with B. melitensis strain 16 M was 1.7x10³-1.7x10(4) cfu/ml. PCR and MRT coincidence was 96%. The diagnostic sensitivity and specificity were determined as 100% and 81.3% respectively for PCR assay and 75% and 75% for MRT. PCR is a useful tool for a fast diagnosis of B. melitensis in sheep milk.
author	Ilhan,Z Solmaz,H Aksakal,A Gulhan,T Ekin,IH Boynukara,B
author_facet	Ilhan,Z Solmaz,H Aksakal,A Gulhan,T Ekin,IH Boynukara,B
author_sort	Ilhan,Z
title	Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay
title_short	Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay
title_full	Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay
title_fullStr	Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay
title_full_unstemmed	Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay
title_sort	detection of brucella melitensis dna in the milk of sheep after abortion by pcr assay
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2008000200005
work_keys_str_mv	AT ilhanz detectionofbrucellamelitensisdnainthemilkofsheepafterabortionbypcrassay AT solmazh detectionofbrucellamelitensisdnainthemilkofsheepafterabortionbypcrassay AT aksakala detectionofbrucellamelitensisdnainthemilkofsheepafterabortionbypcrassay AT gulhant detectionofbrucellamelitensisdnainthemilkofsheepafterabortionbypcrassay AT ekinih detectionofbrucellamelitensisdnainthemilkofsheepafterabortionbypcrassay AT boynukarab detectionofbrucellamelitensisdnainthemilkofsheepafterabortionbypcrassay
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Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay

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