Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile

The efficacy of the disinfectant Duplalim®, a synergistic blend of superquats and glutaraldehyde, was analysed in vitro against 4 fish pathogens. All concentrations tested (1:200 to 1:400 dilutions) were efficacious on killing Vibrio ordalii and Vibrio anguillarum in seawater after 30 s, being the l...

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Autores principales:	Muller,M, Ilardi,P, Avendaño-Herrera,R
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2011
Materias:	disinfectant Chilean fish pathogens Atlantic salmon
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2011000100010
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spelling	oai:scielo:S0301-732X20110001000102011-07-05Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in ChileMuller,MIlardi,PAvendaño-Herrera,R disinfectant Chilean fish pathogens Atlantic salmon The efficacy of the disinfectant Duplalim®, a synergistic blend of superquats and glutaraldehyde, was analysed in vitro against 4 fish pathogens. All concentrations tested (1:200 to 1:400 dilutions) were efficacious on killing Vibrio ordalii and Vibrio anguillarum in seawater after 30 s, being the level of reduction equal to 1.8 x 10(6) CFU / ml. Higher concentration of Duplalim® (1:50 dilutions) and time of exposure (at least 5 min) is needed to kill completely Francisella sp, an intracellular freshwater pathogen. When Infectious Pancreatic Necrosis Virus (IPNV) was treated with 1:400 disinfectant dilution, this concentration had a significant effect after 2 minutes, regardless of the IPNV titres employed (concentration greater than 10(7.6) TCID50/ ml). Duplalim® was tested in large scale against Vibrionaceae members. In comparison to the controls (without the disinfectant), 1:400 dilutions of Duplalim® totally killed V. ordalii and V. anguillarum in seawater as well as on the surface of the fishing net (used in the cages of cultured salmon) after 15 min. Cultivable bacteria remained constant in the buckets without the disinfectant (1.4 ± 0.3 × 10(6) CFU / ml), regardless of the period sampled. In the case of the adherence on the fishing net, bacteria not exposed to the disinfectant were detected at a concentration of 7.6 ± 3.2 × 10(5) CFU / ml. These data indicate that the use of Duplalim® against V. ordalii, V. anguillarum and IPNV is effective in low concentration and short time of exposure (15 min at a concentration of 1:400 dilutions), while the intracellular pathogen requires higher concentration.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.43 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2011000100010en10.4067/S0301-732X2011000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	disinfectant Chilean fish pathogens Atlantic salmon
spellingShingle	disinfectant Chilean fish pathogens Atlantic salmon Muller,M Ilardi,P Avendaño-Herrera,R Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile
description	The efficacy of the disinfectant Duplalim®, a synergistic blend of superquats and glutaraldehyde, was analysed in vitro against 4 fish pathogens. All concentrations tested (1:200 to 1:400 dilutions) were efficacious on killing Vibrio ordalii and Vibrio anguillarum in seawater after 30 s, being the level of reduction equal to 1.8 x 10(6) CFU / ml. Higher concentration of Duplalim® (1:50 dilutions) and time of exposure (at least 5 min) is needed to kill completely Francisella sp, an intracellular freshwater pathogen. When Infectious Pancreatic Necrosis Virus (IPNV) was treated with 1:400 disinfectant dilution, this concentration had a significant effect after 2 minutes, regardless of the IPNV titres employed (concentration greater than 10(7.6) TCID50/ ml). Duplalim® was tested in large scale against Vibrionaceae members. In comparison to the controls (without the disinfectant), 1:400 dilutions of Duplalim® totally killed V. ordalii and V. anguillarum in seawater as well as on the surface of the fishing net (used in the cages of cultured salmon) after 15 min. Cultivable bacteria remained constant in the buckets without the disinfectant (1.4 ± 0.3 × 10(6) CFU / ml), regardless of the period sampled. In the case of the adherence on the fishing net, bacteria not exposed to the disinfectant were detected at a concentration of 7.6 ± 3.2 × 10(5) CFU / ml. These data indicate that the use of Duplalim® against V. ordalii, V. anguillarum and IPNV is effective in low concentration and short time of exposure (15 min at a concentration of 1:400 dilutions), while the intracellular pathogen requires higher concentration.
author	Muller,M Ilardi,P Avendaño-Herrera,R
author_facet	Muller,M Ilardi,P Avendaño-Herrera,R
author_sort	Muller,M
title	Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile
title_short	Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile
title_full	Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile
title_fullStr	Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile
title_full_unstemmed	Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile
title_sort	efficacy of a commercial disinfectant against vibrio ordalii, vibrio anguillarum, francisella sp. and infectious pancreatic necrosis virus (ipnv) pathogens of atlantic salmon (salmo salar) farmed in chile
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2011000100010
work_keys_str_mv	AT mullerm efficacyofacommercialdisinfectantagainstvibrioordaliivibrioanguillarumfrancisellaspandinfectiouspancreaticnecrosisvirusipnvpathogensofatlanticsalmonsalmosalarfarmedinchile AT ilardip efficacyofacommercialdisinfectantagainstvibrioordaliivibrioanguillarumfrancisellaspandinfectiouspancreaticnecrosisvirusipnvpathogensofatlanticsalmonsalmosalarfarmedinchile AT avendanoherrerar efficacyofacommercialdisinfectantagainstvibrioordaliivibrioanguillarumfrancisellaspandinfectiouspancreaticnecrosisvirusipnvpathogensofatlanticsalmonsalmosalarfarmedinchile
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Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile

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