Microsatellite loci and paternity analysis in Nubia and Boer goats
SRCRSP (Small Ruminant Collaborative Research Support Program) loci are polymorphic 100-300 base pair-long microsatellite sequences of repeated dinucleotides that can be amplified by the polymerase chain reaction (PCR). Alleles of a given locus are the PCR products of unique length amplified with a...
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| Autores principales: | , , , , , |
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| Lenguaje: | English |
| Publicado: |
Facultad de Ciencias Veterinarias, Universidad Austral de Chile
2012
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200005 |
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| Sumario: | SRCRSP (Small Ruminant Collaborative Research Support Program) loci are polymorphic 100-300 base pair-long microsatellite sequences of repeated dinucleotides that can be amplified by the polymerase chain reaction (PCR). Alleles of a given locus are the PCR products of unique length amplified with a set of specific primers. SRCRSP loci have been used to identify individuals but not to assess paternity in goats. We have developed a method to assess caprine paternity by polymorphism analysis of SRCRSP loci in a herd of 20 goats from two breeds, nine Nubia and 11 Boer, respectively. Thirty six alleles were amplified: 6 SRCRSP-1; 5 SRCRSP-2; 4 SRCRSP-3; 5 SRCRSP-4; 5 SRCRSP-5; 6 SRCRSP-6 and 5 SRCRSP-9. four alleles appeared only in the Nubia group and eight only in the Boer group. Polymorphism analysis led to a 0.995 certainty for correct exclusion of the second parent. The loci described in this work could be used to control goat paternity as well as to identify individuals. |
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