Microsatellite loci and paternity analysis in Nubia and Boer goats

SRCRSP (Small Ruminant Collaborative Research Support Program) loci are polymorphic 100-300 base pair-long microsatellite sequences of repeated dinucleotides that can be amplified by the polymerase chain reaction (PCR). Alleles of a given locus are the PCR products of unique length amplified with a...

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Autores principales:	Luna-González,A, Hernández-Arteaga,S, Sánchez-Garza,M, López-Revilla,R, Medina-Esparza,L, Cruz-Vázquez,C
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2012
Materias:	goats microsatellites paternity SRCRSP alleles
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200005
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id	oai:scielo:S0301-732X2012000200005
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spelling	oai:scielo:S0301-732X20120002000052013-11-06Microsatellite loci and paternity analysis in Nubia and Boer goatsLuna-González,AHernández-Arteaga,SSánchez-Garza,MLópez-Revilla,RMedina-Esparza,LCruz-Vázquez,C goats microsatellites paternity SRCRSP alleles SRCRSP (Small Ruminant Collaborative Research Support Program) loci are polymorphic 100-300 base pair-long microsatellite sequences of repeated dinucleotides that can be amplified by the polymerase chain reaction (PCR). Alleles of a given locus are the PCR products of unique length amplified with a set of specific primers. SRCRSP loci have been used to identify individuals but not to assess paternity in goats. We have developed a method to assess caprine paternity by polymorphism analysis of SRCRSP loci in a herd of 20 goats from two breeds, nine Nubia and 11 Boer, respectively. Thirty six alleles were amplified: 6 SRCRSP-1; 5 SRCRSP-2; 4 SRCRSP-3; 5 SRCRSP-4; 5 SRCRSP-5; 6 SRCRSP-6 and 5 SRCRSP-9. four alleles appeared only in the Nubia group and eight only in the Boer group. Polymorphism analysis led to a 0.995 certainty for correct exclusion of the second parent. The loci described in this work could be used to control goat paternity as well as to identify individuals.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.44 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200005en10.4067/S0301-732X2012000200005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	goats microsatellites paternity SRCRSP alleles
spellingShingle	goats microsatellites paternity SRCRSP alleles Luna-González,A Hernández-Arteaga,S Sánchez-Garza,M López-Revilla,R Medina-Esparza,L Cruz-Vázquez,C Microsatellite loci and paternity analysis in Nubia and Boer goats
description	SRCRSP (Small Ruminant Collaborative Research Support Program) loci are polymorphic 100-300 base pair-long microsatellite sequences of repeated dinucleotides that can be amplified by the polymerase chain reaction (PCR). Alleles of a given locus are the PCR products of unique length amplified with a set of specific primers. SRCRSP loci have been used to identify individuals but not to assess paternity in goats. We have developed a method to assess caprine paternity by polymorphism analysis of SRCRSP loci in a herd of 20 goats from two breeds, nine Nubia and 11 Boer, respectively. Thirty six alleles were amplified: 6 SRCRSP-1; 5 SRCRSP-2; 4 SRCRSP-3; 5 SRCRSP-4; 5 SRCRSP-5; 6 SRCRSP-6 and 5 SRCRSP-9. four alleles appeared only in the Nubia group and eight only in the Boer group. Polymorphism analysis led to a 0.995 certainty for correct exclusion of the second parent. The loci described in this work could be used to control goat paternity as well as to identify individuals.
author	Luna-González,A Hernández-Arteaga,S Sánchez-Garza,M López-Revilla,R Medina-Esparza,L Cruz-Vázquez,C
author_facet	Luna-González,A Hernández-Arteaga,S Sánchez-Garza,M López-Revilla,R Medina-Esparza,L Cruz-Vázquez,C
author_sort	Luna-González,A
title	Microsatellite loci and paternity analysis in Nubia and Boer goats
title_short	Microsatellite loci and paternity analysis in Nubia and Boer goats
title_full	Microsatellite loci and paternity analysis in Nubia and Boer goats
title_fullStr	Microsatellite loci and paternity analysis in Nubia and Boer goats
title_full_unstemmed	Microsatellite loci and paternity analysis in Nubia and Boer goats
title_sort	microsatellite loci and paternity analysis in nubia and boer goats
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200005
work_keys_str_mv	AT lunagonzaleza microsatellitelociandpaternityanalysisinnubiaandboergoats AT hernandezarteagas microsatellitelociandpaternityanalysisinnubiaandboergoats AT sanchezgarzam microsatellitelociandpaternityanalysisinnubiaandboergoats AT lopezrevillar microsatellitelociandpaternityanalysisinnubiaandboergoats AT medinaesparzal microsatellitelociandpaternityanalysisinnubiaandboergoats AT cruzvazquezc microsatellitelociandpaternityanalysisinnubiaandboergoats
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Microsatellite loci and paternity analysis in Nubia and Boer goats

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