Comparison of two different methods for evaluating boar semen morphology

The objective of this study was to compare the use and effectiveness of two different techniques for assessing sperm morphology, the conventional wet preparation with a of formaldehyde-citrate solution 2.94% and the smear using Bengal Rose dye. 58 ejaculates from eight high performance boars aged be...

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Autores principales:	Oberlender,G, Murgas,LDS, Zangeronimo,MG, Silva,AC, Pereira,LJ, Muzzi,RAL
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2012
Materias:	formaldehyde-citrate Bengal Rose spermatic alterations swine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200016
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spelling	oai:scielo:S0301-732X20120002000162013-11-06Comparison of two different methods for evaluating boar semen morphologyOberlender,GMurgas,LDSZangeronimo,MGSilva,ACPereira,LJMuzzi,RAL formaldehyde-citrate Bengal Rose spermatic alterations swine The objective of this study was to compare the use and effectiveness of two different techniques for assessing sperm morphology, the conventional wet preparation with a of formaldehyde-citrate solution 2.94% and the smear using Bengal Rose dye. 58 ejaculates from eight high performance boars aged between 8 and 25 months were evaluated. The sperm morphology was evaluated after collection of each ejaculate using two techniques: conventional wet preparation (formol citrate solution 2.94%) observed in phase contrast microscopy (1.000X magnification) and a smear prepared with Bengal Rose dye, examined under an optical microscope (400X magnification). It was observed that the percentage of alterations of head, acrosome, middle piece and total morphological changes did not differ (P > 0.05) between the two methods. A higher number of tail defects (P < 0.05) was obtained by using the smear technique with Bengal Rose dye when compared to the conventional technique, this was probably due to the preparation technique of the smear that could result in a greater chance of tail breaking, leading to the visualization of this anomaly. It is concluded that the use of the Bengal Rose dye is an efficient method for evaluating the sperm morphology of boar semen, and thats pecial care should be taken when preparing the smear to avoid affecting the results, mainly regarding tail alterations.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.44 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200016en10.4067/S0301-732X2012000200016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	formaldehyde-citrate Bengal Rose spermatic alterations swine
spellingShingle	formaldehyde-citrate Bengal Rose spermatic alterations swine Oberlender,G Murgas,LDS Zangeronimo,MG Silva,AC Pereira,LJ Muzzi,RAL Comparison of two different methods for evaluating boar semen morphology
description	The objective of this study was to compare the use and effectiveness of two different techniques for assessing sperm morphology, the conventional wet preparation with a of formaldehyde-citrate solution 2.94% and the smear using Bengal Rose dye. 58 ejaculates from eight high performance boars aged between 8 and 25 months were evaluated. The sperm morphology was evaluated after collection of each ejaculate using two techniques: conventional wet preparation (formol citrate solution 2.94%) observed in phase contrast microscopy (1.000X magnification) and a smear prepared with Bengal Rose dye, examined under an optical microscope (400X magnification). It was observed that the percentage of alterations of head, acrosome, middle piece and total morphological changes did not differ (P > 0.05) between the two methods. A higher number of tail defects (P < 0.05) was obtained by using the smear technique with Bengal Rose dye when compared to the conventional technique, this was probably due to the preparation technique of the smear that could result in a greater chance of tail breaking, leading to the visualization of this anomaly. It is concluded that the use of the Bengal Rose dye is an efficient method for evaluating the sperm morphology of boar semen, and thats pecial care should be taken when preparing the smear to avoid affecting the results, mainly regarding tail alterations.
author	Oberlender,G Murgas,LDS Zangeronimo,MG Silva,AC Pereira,LJ Muzzi,RAL
author_facet	Oberlender,G Murgas,LDS Zangeronimo,MG Silva,AC Pereira,LJ Muzzi,RAL
author_sort	Oberlender,G
title	Comparison of two different methods for evaluating boar semen morphology
title_short	Comparison of two different methods for evaluating boar semen morphology
title_full	Comparison of two different methods for evaluating boar semen morphology
title_fullStr	Comparison of two different methods for evaluating boar semen morphology
title_full_unstemmed	Comparison of two different methods for evaluating boar semen morphology
title_sort	comparison of two different methods for evaluating boar semen morphology
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2012000200016
work_keys_str_mv	AT oberlenderg comparisonoftwodifferentmethodsforevaluatingboarsemenmorphology AT murgaslds comparisonoftwodifferentmethodsforevaluatingboarsemenmorphology AT zangeronimomg comparisonoftwodifferentmethodsforevaluatingboarsemenmorphology AT silvaac comparisonoftwodifferentmethodsforevaluatingboarsemenmorphology AT pereiralj comparisonoftwodifferentmethodsforevaluatingboarsemenmorphology AT muzziral comparisonoftwodifferentmethodsforevaluatingboarsemenmorphology
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Comparison of two different methods for evaluating boar semen morphology

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