Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry

The EROD-H4IIE cell bioassay is a screening method for the detection of planar halogenated hydrocarbons (PHH), it has several advantages over analytical chemistry like speed, simplicity, accuracy, high sensitivity and low cost which makes it a valuable bioassay for biomonitoring studies. It has been...

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Autores principales:	Valdovinos,CE, Sotomayor,P, Stech,R, Schoffer,JT, Bustos-López,C
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2013
Materias:	dioxins EROD/H4IIE HRGC/HRMS pork
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2013000200009
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spelling	oai:scielo:S0301-732X20130002000092013-09-03Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometryValdovinos,CESotomayor,PStech,RSchoffer,JTBustos-López,C dioxins EROD/H4IIE HRGC/HRMS pork The EROD-H4IIE cell bioassay is a screening method for the detection of planar halogenated hydrocarbons (PHH), it has several advantages over analytical chemistry like speed, simplicity, accuracy, high sensitivity and low cost which makes it a valuable bioassay for biomonitoring studies. It has been successfully used as screening method for the detection of dioxins and furans (PCDD/Fs) in meat from broiler chicken. The aim of this study was to detect PCDD/Fs in pork, by using both the bioassay and the high resolution gas chromatography/high resolution mass spectrometry (HRGC/ HRMS). 59 composite samples of pork chuck loin were taken at a slaughtering plant of 6 Chilean production facilities between 2004 and 2007, with one sample being taken in 2011. Mean concentrations of PCDD/Fs obtained by HRGC/HRMS ranged between 0.22 and 0.34 pg WHO-TEQ/g fat. The maximum was 0.71 pg WHO-TEQ/g fat. The average value of the congeners was 0.1 pg WHO-TEQ fat-2,3,4,7,8-PeCDF (between 0.03 and 0.28 pg/g fat). Therefore, the samples did not exceed the maximum allowed by national and international legislation. When comparing the results obtained with the two methods, 36 out of 59 sample results were overestimated by the bioassay, while 19 were underestimated and 4 were similar. 15 samples exceeded 1 pg/g TCDD-EQ tissue and only 5 exceeded 2 pg TCDD-EQ/g tissue. It was not possible to establish an equivalence between EROD-H4IIE and HRGC/ HRMS for the detection of PCDD/Fs in pork since there was not a significant association between variables (r = -0.142, P ≥ 0.2840).info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.45 n.2 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2013000200009en10.4067/S0301-732X2013000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	dioxins EROD/H4IIE HRGC/HRMS pork
spellingShingle	dioxins EROD/H4IIE HRGC/HRMS pork Valdovinos,CE Sotomayor,P Stech,R Schoffer,JT Bustos-López,C Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
description	The EROD-H4IIE cell bioassay is a screening method for the detection of planar halogenated hydrocarbons (PHH), it has several advantages over analytical chemistry like speed, simplicity, accuracy, high sensitivity and low cost which makes it a valuable bioassay for biomonitoring studies. It has been successfully used as screening method for the detection of dioxins and furans (PCDD/Fs) in meat from broiler chicken. The aim of this study was to detect PCDD/Fs in pork, by using both the bioassay and the high resolution gas chromatography/high resolution mass spectrometry (HRGC/ HRMS). 59 composite samples of pork chuck loin were taken at a slaughtering plant of 6 Chilean production facilities between 2004 and 2007, with one sample being taken in 2011. Mean concentrations of PCDD/Fs obtained by HRGC/HRMS ranged between 0.22 and 0.34 pg WHO-TEQ/g fat. The maximum was 0.71 pg WHO-TEQ/g fat. The average value of the congeners was 0.1 pg WHO-TEQ fat-2,3,4,7,8-PeCDF (between 0.03 and 0.28 pg/g fat). Therefore, the samples did not exceed the maximum allowed by national and international legislation. When comparing the results obtained with the two methods, 36 out of 59 sample results were overestimated by the bioassay, while 19 were underestimated and 4 were similar. 15 samples exceeded 1 pg/g TCDD-EQ tissue and only 5 exceeded 2 pg TCDD-EQ/g tissue. It was not possible to establish an equivalence between EROD-H4IIE and HRGC/ HRMS for the detection of PCDD/Fs in pork since there was not a significant association between variables (r = -0.142, P ≥ 0.2840).
author	Valdovinos,CE Sotomayor,P Stech,R Schoffer,JT Bustos-López,C
author_facet	Valdovinos,CE Sotomayor,P Stech,R Schoffer,JT Bustos-López,C
author_sort	Valdovinos,CE
title	Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
title_short	Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
title_full	Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
title_fullStr	Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
title_full_unstemmed	Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
title_sort	application of the erod-h4iie bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2013000200009
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Application of the EROD-H4IIE bioassay for the determination of dioxins in pork in comparison to high resolution gas chromatography coupled to high resolution mass spectrometry

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