A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice

Brucella species are facultative intracellular gram-negative bacteria that can multiply within phagocytic and non-phagocytic cells of humans or animals as end hosts. B. melitensis causes abortion in pregnant animals and undulant fever in humans. A 41 kDa surface protein (sp41) is associated with bac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Al-Mariri,A, Akel,R, Abbady,AQ
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2014
Materias:	DNA vaccine Brucella protein protective
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2014000100008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0301-732X2014000100008
record_format	dspace
spelling	oai:scielo:S0301-732X20140001000082015-10-30A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c miceAl-Mariri,AAkel,RAbbady,AQ DNA vaccine Brucella protein protective Brucella species are facultative intracellular gram-negative bacteria that can multiply within phagocytic and non-phagocytic cells of humans or animals as end hosts. B. melitensis causes abortion in pregnant animals and undulant fever in humans. A 41 kDa surface protein (sp41) is associated with bacterial adherence and invasion of HeLa cells. The role of this protein a is important for the interaction with host cells. Previously, the putative periplasmic binding protein p39 had been described as T-cell immunodominant Brucella antigens. Both vectors (pCIp39 and pCIsp41) induced antigen-specific serum immunoglobulin as well as a T-cell-proliferative response and a strong gamma interferon production upon re-stimulation with either the specific antigens or Brucella extract. The level of protection was significant in pCIp39 and pCIsp41 treated mice but it was lower than the required level.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.46 n.1 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2014000100008en10.4067/S0301-732X2014000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA vaccine Brucella protein protective
spellingShingle	DNA vaccine Brucella protein protective Al-Mariri,A Akel,R Abbady,AQ A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice
description	Brucella species are facultative intracellular gram-negative bacteria that can multiply within phagocytic and non-phagocytic cells of humans or animals as end hosts. B. melitensis causes abortion in pregnant animals and undulant fever in humans. A 41 kDa surface protein (sp41) is associated with bacterial adherence and invasion of HeLa cells. The role of this protein a is important for the interaction with host cells. Previously, the putative periplasmic binding protein p39 had been described as T-cell immunodominant Brucella antigens. Both vectors (pCIp39 and pCIsp41) induced antigen-specific serum immunoglobulin as well as a T-cell-proliferative response and a strong gamma interferon production upon re-stimulation with either the specific antigens or Brucella extract. The level of protection was significant in pCIp39 and pCIsp41 treated mice but it was lower than the required level.
author	Al-Mariri,A Akel,R Abbady,AQ
author_facet	Al-Mariri,A Akel,R Abbady,AQ
author_sort	Al-Mariri,A
title	A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice
title_short	A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice
title_full	A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice
title_fullStr	A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice
title_full_unstemmed	A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice
title_sort	dna vaccine encoding p39 and sp41 of brucella melitensis induces protective immunity in balb/c mice
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2014000100008
work_keys_str_mv	AT almariria adnavaccineencodingp39andsp41ofbrucellamelitensisinducesprotectiveimmunityinbalbcmice AT akelr adnavaccineencodingp39andsp41ofbrucellamelitensisinducesprotectiveimmunityinbalbcmice AT abbadyaq adnavaccineencodingp39andsp41ofbrucellamelitensisinducesprotectiveimmunityinbalbcmice AT almariria dnavaccineencodingp39andsp41ofbrucellamelitensisinducesprotectiveimmunityinbalbcmice AT akelr dnavaccineencodingp39andsp41ofbrucellamelitensisinducesprotectiveimmunityinbalbcmice AT abbadyaq dnavaccineencodingp39andsp41ofbrucellamelitensisinducesprotectiveimmunityinbalbcmice
_version_	1718437927521878016

A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice

Ejemplares similares