Identification of Nosema ceranae in the Valparaíso District, Chile
This study aimed to demonstrate for the first time the presence of Nosema ceranae in the Valparaíso District. The analised bee samples were obtained in the Valparaíso Region, between 2011 and 2012, from beehives located in San Antonio, Limache and La Ligua. Samples obtained from groups of 60 bees we...
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| Lenguaje: | English |
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Facultad de Ciencias Veterinarias, Universidad Austral de Chile
2014
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oai:scielo:S0301-732X20140003000212015-01-14Identification of Nosema ceranae in the Valparaíso District, ChileBravo,JCarbonell,VValdebenito,JTFigueroa,CValdovinos,CEMartín-Hernández,RHiges,MDelporte,C Nosema ceranae Apis mellifera Nosema apis Nosemosis This study aimed to demonstrate for the first time the presence of Nosema ceranae in the Valparaíso District. The analised bee samples were obtained in the Valparaíso Region, between 2011 and 2012, from beehives located in San Antonio, Limache and La Ligua. Samples obtained from groups of 60 bees were macerated and analysed by phase contrast microscopy at 40x. Then, DNA was extracted and the gene that encodes for 16SrRNA of Nosema apis and N. ceranae was amplified by multiplex PCR. 100% of the samples were infected by N. ceranae, while N. apis was not detected. During the period of study 2,915 colonies died in the Valparaíso Region which is 47.3% of the total hives of the Region. The loss of beehives during this period in the V Region of Chile could be related to either the lack of training in management and health monitoring of the colonies or the presence of N. ceranae.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.46 n.3 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2014000300021en10.4067/S0301-732X2014000300021 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Nosema ceranae Apis mellifera Nosema apis Nosemosis |
| spellingShingle |
Nosema ceranae Apis mellifera Nosema apis Nosemosis Bravo,J Carbonell,V Valdebenito,JT Figueroa,C Valdovinos,CE Martín-Hernández,R Higes,M Delporte,C Identification of Nosema ceranae in the Valparaíso District, Chile |
| description |
This study aimed to demonstrate for the first time the presence of Nosema ceranae in the Valparaíso District. The analised bee samples were obtained in the Valparaíso Region, between 2011 and 2012, from beehives located in San Antonio, Limache and La Ligua. Samples obtained from groups of 60 bees were macerated and analysed by phase contrast microscopy at 40x. Then, DNA was extracted and the gene that encodes for 16SrRNA of Nosema apis and N. ceranae was amplified by multiplex PCR. 100% of the samples were infected by N. ceranae, while N. apis was not detected. During the period of study 2,915 colonies died in the Valparaíso Region which is 47.3% of the total hives of the Region. The loss of beehives during this period in the V Region of Chile could be related to either the lack of training in management and health monitoring of the colonies or the presence of N. ceranae. |
| author |
Bravo,J Carbonell,V Valdebenito,JT Figueroa,C Valdovinos,CE Martín-Hernández,R Higes,M Delporte,C |
| author_facet |
Bravo,J Carbonell,V Valdebenito,JT Figueroa,C Valdovinos,CE Martín-Hernández,R Higes,M Delporte,C |
| author_sort |
Bravo,J |
| title |
Identification of Nosema ceranae in the Valparaíso District, Chile |
| title_short |
Identification of Nosema ceranae in the Valparaíso District, Chile |
| title_full |
Identification of Nosema ceranae in the Valparaíso District, Chile |
| title_fullStr |
Identification of Nosema ceranae in the Valparaíso District, Chile |
| title_full_unstemmed |
Identification of Nosema ceranae in the Valparaíso District, Chile |
| title_sort |
identification of nosema ceranae in the valparaíso district, chile |
| publisher |
Facultad de Ciencias Veterinarias, Universidad Austral de Chile |
| publishDate |
2014 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2014000300021 |
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