Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study

Cryptosporidium spp. is a globally distributed protozoan that causes digestive disease in different animals including humans. Excreted oocysts contaminate water and soil, constituting a public health threat. Sensitive and fast methods to detect oocysts in water samples are necessary due to the small...

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Autores principales:	Díaz-Lee,A, Molina,R, Dougnac,C, Mercado,R, Retamal,P, Fredes,F
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2015
Materias:	Cryptosporidium water diagnosis Ziehl-Neelsen Auramine nested PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000100015
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spelling	oai:scielo:S0301-732X20150001000152016-07-04Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary studyDíaz-Lee,AMolina,RDougnac,CMercado,RRetamal,PFredes,F Cryptosporidium water diagnosis Ziehl-Neelsen Auramine nested PCR Cryptosporidium spp. is a globally distributed protozoan that causes digestive disease in different animals including humans. Excreted oocysts contaminate water and soil, constituting a public health threat. Sensitive and fast methods to detect oocysts in water samples are necessary due to the small number of oocysts present in the environment and their low infectious dose. This study compared the analytical sensitivity of two staining techniques, modified Ziehl-Neelsen and Auramine versus a nested PCR that amplifies a region of ~520bp from 18S rDNA gene, to detect Cryptosporidium spp. in water samples. Water was inoculated with oocysts using serial dilutions, and then a water filtration method was used to recover the parasite oocysts. The staining techniques had similar analytical sensitivity, detecting 8 oocysts/mL, while the nested PCR detected down to 6 oocysts/mL. In conclusion, all of these methods are effective for Cryptosporidium spp. detection in water samples, contributing to the implementation of standardized diagnostic methods for environmental water infectious agents.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.47 n.1 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000100015en10.4067/S0301-732X2015000100015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cryptosporidium water diagnosis Ziehl-Neelsen Auramine nested PCR
spellingShingle	Cryptosporidium water diagnosis Ziehl-Neelsen Auramine nested PCR Díaz-Lee,A Molina,R Dougnac,C Mercado,R Retamal,P Fredes,F Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
description	Cryptosporidium spp. is a globally distributed protozoan that causes digestive disease in different animals including humans. Excreted oocysts contaminate water and soil, constituting a public health threat. Sensitive and fast methods to detect oocysts in water samples are necessary due to the small number of oocysts present in the environment and their low infectious dose. This study compared the analytical sensitivity of two staining techniques, modified Ziehl-Neelsen and Auramine versus a nested PCR that amplifies a region of ~520bp from 18S rDNA gene, to detect Cryptosporidium spp. in water samples. Water was inoculated with oocysts using serial dilutions, and then a water filtration method was used to recover the parasite oocysts. The staining techniques had similar analytical sensitivity, detecting 8 oocysts/mL, while the nested PCR detected down to 6 oocysts/mL. In conclusion, all of these methods are effective for Cryptosporidium spp. detection in water samples, contributing to the implementation of standardized diagnostic methods for environmental water infectious agents.
author	Díaz-Lee,A Molina,R Dougnac,C Mercado,R Retamal,P Fredes,F
author_facet	Díaz-Lee,A Molina,R Dougnac,C Mercado,R Retamal,P Fredes,F
author_sort	Díaz-Lee,A
title	Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
title_short	Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
title_full	Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
title_fullStr	Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
title_full_unstemmed	Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
title_sort	analytical sensitivity of staining and molecular techniques for the detection of cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000100015
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Analytical sensitivity of staining and molecular techniques for the detection of Cryptosporidium spp. oocysts isolated from bovines in water samples: a preliminary study

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