Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system

Diagnosis of Mycobacterium avium subsp. paratuberculosis infection by liquid culture is sensitive, faster than conventional solid culture and automated. However, a disadvantage of these culture systems is the potential for high frequency of culture contamination. Contaminant bacteria were identified...

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Autores principales:	Steuer,P, de Waard,J, Collins,MT, Troncoso,EP, Martínez,OA, Tejeda,C, Salgado,MA
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2015
Materias:	Mycobacterium avium subsp. paratuberculosis liquid culture contaminant bacteria RISA PRA-hps65
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000100016
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spelling	oai:scielo:S0301-732X20150001000162016-07-04Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 systemSteuer,Pde Waard,JCollins,MTTroncoso,EPMartínez,OATejeda,CSalgado,MA Mycobacterium avium subsp. paratuberculosis liquid culture contaminant bacteria RISA PRA-hps65 Diagnosis of Mycobacterium avium subsp. paratuberculosis infection by liquid culture is sensitive, faster than conventional solid culture and automated. However, a disadvantage of these culture systems is the potential for high frequency of culture contamination. Contaminant bacteria were identified as a step toward better contaminant control. No mycobacteria were detected by mycobacterial Polymerase Chain Reaction-Restriction Enzyme Analysis (PRA)-hsp65. Ribosomal Intergenic Spacer Analysis (RISA) followed by sequence analysis identified Paenibacillus sp., Enterobacteriaceae and Pseudomonas aeruginosa as common contaminants. The present study aimed to identify a representative sample of contaminants encountered when culturing clinical faecal samples from Chilean cattle. Further studies involving a larger and more representative sample of animals are required to extrapolate the results to a broader population.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.47 n.1 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000100016en10.4067/S0301-732X2015000100016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mycobacterium avium subsp. paratuberculosis liquid culture contaminant bacteria RISA PRA-hps65
spellingShingle	Mycobacterium avium subsp. paratuberculosis liquid culture contaminant bacteria RISA PRA-hps65 Steuer,P de Waard,J Collins,MT Troncoso,EP Martínez,OA Tejeda,C Salgado,MA Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
description	Diagnosis of Mycobacterium avium subsp. paratuberculosis infection by liquid culture is sensitive, faster than conventional solid culture and automated. However, a disadvantage of these culture systems is the potential for high frequency of culture contamination. Contaminant bacteria were identified as a step toward better contaminant control. No mycobacteria were detected by mycobacterial Polymerase Chain Reaction-Restriction Enzyme Analysis (PRA)-hsp65. Ribosomal Intergenic Spacer Analysis (RISA) followed by sequence analysis identified Paenibacillus sp., Enterobacteriaceae and Pseudomonas aeruginosa as common contaminants. The present study aimed to identify a representative sample of contaminants encountered when culturing clinical faecal samples from Chilean cattle. Further studies involving a larger and more representative sample of animals are required to extrapolate the results to a broader population.
author	Steuer,P de Waard,J Collins,MT Troncoso,EP Martínez,OA Tejeda,C Salgado,MA
author_facet	Steuer,P de Waard,J Collins,MT Troncoso,EP Martínez,OA Tejeda,C Salgado,MA
author_sort	Steuer,P
title	Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
title_short	Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
title_full	Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
title_fullStr	Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
title_full_unstemmed	Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
title_sort	identification of contaminating bacteria when attempting to isolate mycobacterium avium subsp. paratuberculosis (map) from bovine faecal and tissue samples using the bactec mgit 960 system
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000100016
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Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system

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