Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis

The skin test with purified protein derivatives (PPD) from Mycobacterium bovis has been used for the diagnosis of bovine tuberculosis. However, due to the suboptimal specificity of this protein mixture, improved tests based on defined specific antigens are needed. In the present study, recombinant p...

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Autores principales:	Melo,ESP, Souza,IIF, Ramos,CAN, Osório,ALAR, Verbisck,NV, Araújo,FR
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2015
Materias:	bovine tuberculosis recombinant proteins skin test EsxI PE5
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000300003
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spelling	oai:scielo:S0301-732X20150003000032016-03-21Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosisMelo,ESPSouza,IIFRamos,CANOsório,ALARVerbisck,NVAraújo,FR bovine tuberculosis recombinant proteins skin test EsxI PE5 The skin test with purified protein derivatives (PPD) from Mycobacterium bovis has been used for the diagnosis of bovine tuberculosis. However, due to the suboptimal specificity of this protein mixture, improved tests based on defined specific antigens are needed. In the present study, recombinant proteins from M. bovis were evaluated as antigens in the skin test. Among these proteins, EsxI, Mb0143, PE5, and PE13 are antigens tested for the first time in skin test on cattle. Sensitised and non-sensitised cattle to the inactivated AN5 strain of M. bovis were simultaneously injected with each recombinant protein, a cocktail with all recombinant proteins, M. bovis PPD, M. avium PPD and saline. Only the proteins EsxI, Mb0143 and PE5 were able to differentiate sensitised and non-sensitised cattle when 320 irg of protein was used and the reading done 24 hours post-injection. EsxI and PE5 exhibited a sensitivity level of 83.33% and specificity of 100% and 80%, respectively. The results of the present study suggest that the recombinant proteins have potential to be assessed as antigens in skin tests in cattle.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.47 n.3 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000300003en10.4067/S0301-732X2015000300003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bovine tuberculosis recombinant proteins skin test EsxI PE5
spellingShingle	bovine tuberculosis recombinant proteins skin test EsxI PE5 Melo,ESP Souza,IIF Ramos,CAN Osório,ALAR Verbisck,NV Araújo,FR Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
description	The skin test with purified protein derivatives (PPD) from Mycobacterium bovis has been used for the diagnosis of bovine tuberculosis. However, due to the suboptimal specificity of this protein mixture, improved tests based on defined specific antigens are needed. In the present study, recombinant proteins from M. bovis were evaluated as antigens in the skin test. Among these proteins, EsxI, Mb0143, PE5, and PE13 are antigens tested for the first time in skin test on cattle. Sensitised and non-sensitised cattle to the inactivated AN5 strain of M. bovis were simultaneously injected with each recombinant protein, a cocktail with all recombinant proteins, M. bovis PPD, M. avium PPD and saline. Only the proteins EsxI, Mb0143 and PE5 were able to differentiate sensitised and non-sensitised cattle when 320 irg of protein was used and the reading done 24 hours post-injection. EsxI and PE5 exhibited a sensitivity level of 83.33% and specificity of 100% and 80%, respectively. The results of the present study suggest that the recombinant proteins have potential to be assessed as antigens in skin tests in cattle.
author	Melo,ESP Souza,IIF Ramos,CAN Osório,ALAR Verbisck,NV Araújo,FR
author_facet	Melo,ESP Souza,IIF Ramos,CAN Osório,ALAR Verbisck,NV Araújo,FR
author_sort	Melo,ESP
title	Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
title_short	Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
title_full	Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
title_fullStr	Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
title_full_unstemmed	Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
title_sort	evaluation of the use of recombinant proteins of mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000300003
work_keys_str_mv	AT meloesp evaluationoftheuseofrecombinantproteinsofmycobacteriumbovisasantigensinintradermaltestsfordiagnosisofbovinetuberculosis AT souzaiif evaluationoftheuseofrecombinantproteinsofmycobacteriumbovisasantigensinintradermaltestsfordiagnosisofbovinetuberculosis AT ramoscan evaluationoftheuseofrecombinantproteinsofmycobacteriumbovisasantigensinintradermaltestsfordiagnosisofbovinetuberculosis AT osorioalar evaluationoftheuseofrecombinantproteinsofmycobacteriumbovisasantigensinintradermaltestsfordiagnosisofbovinetuberculosis AT verbiscknv evaluationoftheuseofrecombinantproteinsofmycobacteriumbovisasantigensinintradermaltestsfordiagnosisofbovinetuberculosis AT araujofr evaluationoftheuseofrecombinantproteinsofmycobacteriumbovisasantigensinintradermaltestsfordiagnosisofbovinetuberculosis
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Evaluation of the use of recombinant proteins of Mycobacterium bovis as antigens in intradermal tests for diagnosis of bovine tuberculosis

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