Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic incurable infection of intestinal tract of animals. The study took place in 2012, on 491 Korean black goats, older than 2 years, from goat herds located in Southern region, Korea. The animals were sampled and...

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Autores principales: Kim,JM, Pham,LD, Jang,YH, Kim,NR, Ryoo,SY, Jang,YR, Jang,JM, Jung,SC
Lenguaje:English
Publicado: Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2015
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2015000300017
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Sumario:Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic incurable infection of intestinal tract of animals. The study took place in 2012, on 491 Korean black goats, older than 2 years, from goat herds located in Southern region, Korea. The animals were sampled and tested for paratuberculosis. Enzyme-linked immunosorbent assay and microbiological culture were used to investigate MAP infection. Among the 491 goats from 100 herds screened, 4 (0.8%) and 3 (0.6%) goats were defined positive for MAP by ELISA and faecal culture, respectively. Isolated colonies were confirmed as MAP by IS900-PCR, and genotyped by IS1311 PCR-REA assays. The results revealed MAP infection at a low prevalence rate with "cattle type" and "bison type" genotypes.