Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

This study was conducted to investigate the presence of some virulence and antimicrobial resistance genes in E. coli isolates from diarrhoiec dogs in Iran. Seventy dogs were randomly selected by direct sampling. Rectal swabs were collected and cultured for isolation and identification of E. coli fol...

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Autores principales:	Torkan,S, Bahadoranian,MA, Khamesipour,F, Anyanwu,MU
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2016
Materias:	antimicrobial resistance diarrhoea dogs Eschericha coli gene virulence.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000200008
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spelling	oai:scielo:S0301-732X20160002000082016-11-03Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in IranTorkan,SBahadoranian,MAKhamesipour,FAnyanwu,MU antimicrobial resistance diarrhoea dogs Eschericha coli gene virulence. This study was conducted to investigate the presence of some virulence and antimicrobial resistance genes in E. coli isolates from diarrhoiec dogs in Iran. Seventy dogs were randomly selected by direct sampling. Rectal swabs were collected and cultured for isolation and identification of E. coli following standard methods. Polymerase chain reaction (PCR) was used to detect 5 virulence genes and 12 antibacterial resistance genes in 14 of the isolates. From the 70 rectal swabs cultured, 33 (47.1%) gave positive growth of E. coli. Out of 14 isolates tested for the presence of virulence genes, 9 (64.3%) were positive for PCR of stx (1), 5 (35.7%) were positive for stx (2), 7 (50%) were positive for eae, and 1 (7.1%) isolate was positive for cnf (1). Out of the 14 isolates tested for the presence of antibacterial resistance genes, 9 (64.3%) were positive for CITM gene, 6 (42.9%) were positive for aad (A1) and bla (SHV), 5 (35.7%) were positive for tet (A), dfr (A1) and cat (1), 4 (28.6%) were positive for aac (3)-IV, 3 (21.4%) were positive for both tet (B), sul (1) and cml (A), while 1 (7.1%) of the isolate was positive for ere. The results showed that enterohaemorrhagic E. coli (EHEC), shiga toxigenic E. coli (STEC) and necrotoxic E. coli (NTEC) strains harboring several antibacterial resistance genes could be involved in canine diarrhoea in Iran.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.48 n.2 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000200008en10.4067/S0301-732X2016000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antimicrobial resistance diarrhoea dogs Eschericha coli gene virulence.
spellingShingle	antimicrobial resistance diarrhoea dogs Eschericha coli gene virulence. Torkan,S Bahadoranian,MA Khamesipour,F Anyanwu,MU Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran
description	This study was conducted to investigate the presence of some virulence and antimicrobial resistance genes in E. coli isolates from diarrhoiec dogs in Iran. Seventy dogs were randomly selected by direct sampling. Rectal swabs were collected and cultured for isolation and identification of E. coli following standard methods. Polymerase chain reaction (PCR) was used to detect 5 virulence genes and 12 antibacterial resistance genes in 14 of the isolates. From the 70 rectal swabs cultured, 33 (47.1%) gave positive growth of E. coli. Out of 14 isolates tested for the presence of virulence genes, 9 (64.3%) were positive for PCR of stx (1), 5 (35.7%) were positive for stx (2), 7 (50%) were positive for eae, and 1 (7.1%) isolate was positive for cnf (1). Out of the 14 isolates tested for the presence of antibacterial resistance genes, 9 (64.3%) were positive for CITM gene, 6 (42.9%) were positive for aad (A1) and bla (SHV), 5 (35.7%) were positive for tet (A), dfr (A1) and cat (1), 4 (28.6%) were positive for aac (3)-IV, 3 (21.4%) were positive for both tet (B), sul (1) and cml (A), while 1 (7.1%) of the isolate was positive for ere. The results showed that enterohaemorrhagic E. coli (EHEC), shiga toxigenic E. coli (STEC) and necrotoxic E. coli (NTEC) strains harboring several antibacterial resistance genes could be involved in canine diarrhoea in Iran.
author	Torkan,S Bahadoranian,MA Khamesipour,F Anyanwu,MU
author_facet	Torkan,S Bahadoranian,MA Khamesipour,F Anyanwu,MU
author_sort	Torkan,S
title	Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran
title_short	Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran
title_full	Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran
title_fullStr	Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran
title_full_unstemmed	Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran
title_sort	detection of virulence and antimicrobial resistance genes in escherichia coli isolates from diarrhoiec dogs in iran
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000200008
work_keys_str_mv	AT torkans detectionofvirulenceandantimicrobialresistancegenesinescherichiacoliisolatesfromdiarrhoiecdogsiniran AT bahadoranianma detectionofvirulenceandantimicrobialresistancegenesinescherichiacoliisolatesfromdiarrhoiecdogsiniran AT khamesipourf detectionofvirulenceandantimicrobialresistancegenesinescherichiacoliisolatesfromdiarrhoiecdogsiniran AT anyanwumu detectionofvirulenceandantimicrobialresistancegenesinescherichiacoliisolatesfromdiarrhoiecdogsiniran
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Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

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