Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico

Brucellosis is a zoonosis that affects many animal species worldwide. Humans are often infected through direct animal contact, through contact with animal excretions, or through ingestion of unpasteurized dairy products. Livestock manure is extensively used as a pasture fertilizer in many production...

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Autores principales:	Morales-Estrada,AI, Hernández-Castro,R, López-Merino,A, Singh-Bedi,J, Contreras-Rodríguez,A
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2016
Materias:	brucellosis Brucella manure zoonosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000200014
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spelling	oai:scielo:S0301-732X20160002000142016-11-03Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in MexicoMorales-Estrada,AIHernández-Castro,RLópez-Merino,ASingh-Bedi,JContreras-Rodríguez,A brucellosis Brucella manure zoonosis Brucellosis is a zoonosis that affects many animal species worldwide. Humans are often infected through direct animal contact, through contact with animal excretions, or through ingestion of unpasteurized dairy products. Livestock manure is extensively used as a pasture fertilizer in many production systems, especially in non-developed countries. Some microbiological studies warn of the risk of manure being a disseminator vector of pathogenic microorganisms. The present study aimed to isolate Brucella in manure from cows and goats that were serologically positive for brucellosis in an endemic region of Mexico. We then used E-test methods to evaluate the isolated microorganisms' susceptibility to antimicrobial agents in vitro. Brucella isolation was performed via a pre-enriched selective culture, and subcultures using selective agar plates. Isolates were identified using microbiological tests as well as BCSP31 PCR and Bruce-Ladder multiplex PCR. We isolated 10 Brucella spp. strains, all of which amplified the genus-specific gene that encodes the BCSP31 protein. The species were identified using multiplex PCR. Interestingly, B. melitensis, B. abortus, and B. suis were isolated from cow manure, while B. abortus was just isolated from goat manure. The isolates did not include any strains that are routinely vaccinated against. Some isolates were resistant to ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, and rifampicin. Manure from infected animals could represent a vehicle for human exposure to Brucella. More detailed studies are needed to evaluate Brucella-contaminated manure as a risk factor in endemic regions.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.48 n.2 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000200014en10.4067/S0301-732X2016000200014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	brucellosis Brucella manure zoonosis
spellingShingle	brucellosis Brucella manure zoonosis Morales-Estrada,AI Hernández-Castro,R López-Merino,A Singh-Bedi,J Contreras-Rodríguez,A Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico
description	Brucellosis is a zoonosis that affects many animal species worldwide. Humans are often infected through direct animal contact, through contact with animal excretions, or through ingestion of unpasteurized dairy products. Livestock manure is extensively used as a pasture fertilizer in many production systems, especially in non-developed countries. Some microbiological studies warn of the risk of manure being a disseminator vector of pathogenic microorganisms. The present study aimed to isolate Brucella in manure from cows and goats that were serologically positive for brucellosis in an endemic region of Mexico. We then used E-test methods to evaluate the isolated microorganisms' susceptibility to antimicrobial agents in vitro. Brucella isolation was performed via a pre-enriched selective culture, and subcultures using selective agar plates. Isolates were identified using microbiological tests as well as BCSP31 PCR and Bruce-Ladder multiplex PCR. We isolated 10 Brucella spp. strains, all of which amplified the genus-specific gene that encodes the BCSP31 protein. The species were identified using multiplex PCR. Interestingly, B. melitensis, B. abortus, and B. suis were isolated from cow manure, while B. abortus was just isolated from goat manure. The isolates did not include any strains that are routinely vaccinated against. Some isolates were resistant to ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, and rifampicin. Manure from infected animals could represent a vehicle for human exposure to Brucella. More detailed studies are needed to evaluate Brucella-contaminated manure as a risk factor in endemic regions.
author	Morales-Estrada,AI Hernández-Castro,R López-Merino,A Singh-Bedi,J Contreras-Rodríguez,A
author_facet	Morales-Estrada,AI Hernández-Castro,R López-Merino,A Singh-Bedi,J Contreras-Rodríguez,A
author_sort	Morales-Estrada,AI
title	Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico
title_short	Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico
title_full	Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico
title_fullStr	Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico
title_full_unstemmed	Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico
title_sort	isolation, identification, and antimicrobial susceptibility of brucella spp. cultured from cows and goats manure in mexico
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000200014
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Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico

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