Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits

The aim was to investigate the haematological and blood biochemistry changes of adult rabbits after two intravenous administrations of low-doses of E. coli lipopolysacharide (LPS). Six clinically healthy rabbits were distributed through a cross over design to experimental groups: Group 1 (LPS) treat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peñailillo,AK, Sepulveda,MA, Palma,CJ, Espinoza,A, Aguilera,M, Burgos,RA, Carretta,D, Islas,A, Pérez,R
Lenguaje:	English
Publicado:	Facultad de Ciencias Veterinarias, Universidad Austral de Chile 2016
Materias:	lipopolysaccharide haematology blood biochemistry rabbits
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000300012
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0301-732X2016000300012
record_format	dspace
spelling	oai:scielo:S0301-732X20160003000122016-12-27Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbitsPeñailillo,AKSepulveda,MAPalma,CJEspinoza,AAguilera,MBurgos,RACarretta,DIslas,APérez,R lipopolysaccharide haematology blood biochemistry rabbits The aim was to investigate the haematological and blood biochemistry changes of adult rabbits after two intravenous administrations of low-doses of E. coli lipopolysacharide (LPS). Six clinically healthy rabbits were distributed through a cross over design to experimental groups: Group 1 (LPS) treated with two intravenous doses of 1 μg/kg bw of E. coli LPS at 6 h intervals. Group 2 (Control) treated with an equivalent volume of saline solution (SS) at similar intervals. Rectal temperature (T°R), serum concentrations of interleukin-6 (IL-6), C-reactive protein (CRP), haemogram, and the activity of enzymes aspartate aminotransferase (AST) and gamma glutamil transferase (GGT) were measured before and after LPS or SS administration. The administration of LPS in rabbits induced significant increases in IL-6 and CRP concentrations. Leukopenia associated to significant decreases of monocytes, lymphocytes and platelet count, with no significant changes in neutrophil count, were observed at 4 and 8 h after LPS administration. There were significant increases in total leukocytes and neutrophils count at 12 and 24 h. No significant changes (P > 0.05) were observed in mean values of PCV, total proteins, albumin and in the activity of enzymes AST and GGT. The administration of low doses of E. coli LPS induces a fast increase in body temperature, haematological and biochemical shifts, which are milder and have a lower intensity and shorter than previous reports in rabbits treated with high doses of LPS. These changes seem to be consistent with an initial inflammatory response induced by a gram-negative infection.info:eu-repo/semantics/openAccessFacultad de Ciencias Veterinarias, Universidad Austral de ChileArchivos de medicina veterinaria v.48 n.3 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000300012en10.4067/S0301-732X2016000300012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	lipopolysaccharide haematology blood biochemistry rabbits
spellingShingle	lipopolysaccharide haematology blood biochemistry rabbits Peñailillo,AK Sepulveda,MA Palma,CJ Espinoza,A Aguilera,M Burgos,RA Carretta,D Islas,A Pérez,R Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits
description	The aim was to investigate the haematological and blood biochemistry changes of adult rabbits after two intravenous administrations of low-doses of E. coli lipopolysacharide (LPS). Six clinically healthy rabbits were distributed through a cross over design to experimental groups: Group 1 (LPS) treated with two intravenous doses of 1 μg/kg bw of E. coli LPS at 6 h intervals. Group 2 (Control) treated with an equivalent volume of saline solution (SS) at similar intervals. Rectal temperature (T°R), serum concentrations of interleukin-6 (IL-6), C-reactive protein (CRP), haemogram, and the activity of enzymes aspartate aminotransferase (AST) and gamma glutamil transferase (GGT) were measured before and after LPS or SS administration. The administration of LPS in rabbits induced significant increases in IL-6 and CRP concentrations. Leukopenia associated to significant decreases of monocytes, lymphocytes and platelet count, with no significant changes in neutrophil count, were observed at 4 and 8 h after LPS administration. There were significant increases in total leukocytes and neutrophils count at 12 and 24 h. No significant changes (P > 0.05) were observed in mean values of PCV, total proteins, albumin and in the activity of enzymes AST and GGT. The administration of low doses of E. coli LPS induces a fast increase in body temperature, haematological and biochemical shifts, which are milder and have a lower intensity and shorter than previous reports in rabbits treated with high doses of LPS. These changes seem to be consistent with an initial inflammatory response induced by a gram-negative infection.
author	Peñailillo,AK Sepulveda,MA Palma,CJ Espinoza,A Aguilera,M Burgos,RA Carretta,D Islas,A Pérez,R
author_facet	Peñailillo,AK Sepulveda,MA Palma,CJ Espinoza,A Aguilera,M Burgos,RA Carretta,D Islas,A Pérez,R
author_sort	Peñailillo,AK
title	Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits
title_short	Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits
title_full	Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits
title_fullStr	Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits
title_full_unstemmed	Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits
title_sort	haematological and blood biochemical changes induced by the administration of low doses of escherichia coli lipopolysaccharide in rabbits
publisher	Facultad de Ciencias Veterinarias, Universidad Austral de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X2016000300012
work_keys_str_mv	AT penaililloak haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT sepulvedama haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT palmacj haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT espinozaa haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT aguileram haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT burgosra haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT carrettad haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT islasa haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits AT perezr haematologicalandbloodbiochemicalchangesinducedbytheadministrationoflowdosesofescherichiacolilipopolysaccharideinrabbits
_version_	1718437971154173952

Haematological and blood biochemical changes induced by the administration of low doses of Escherichia coli lipopolysaccharide in rabbits

Ejemplares similares