Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain

The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude p...

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Autores principales: Cozzolino,Daniel, Delucchi,Inés, Kholi,Moham, Vázquez,Daniel
Lenguaje:English
Publicado: Instituto de Investigaciones Agropecuarias, INIA 2006
Materias:
NIR
SDS
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072006000400005
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spelling oai:scielo:S0365-280720060004000052007-01-17Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat GrainCozzolino,DanielDelucchi,InésKholi,MohamVázquez,Daniel whole wheat NIR protein wet gluten grain quality SDS The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAAgricultura Técnica v.66 n.4 20062006-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072006000400005en10.4067/S0365-28072006000400005
institution Scielo Chile
collection Scielo Chile
language English
topic whole wheat
NIR
protein
wet gluten
grain quality
SDS
spellingShingle whole wheat
NIR
protein
wet gluten
grain quality
SDS
Cozzolino,Daniel
Delucchi,Inés
Kholi,Moham
Vázquez,Daniel
Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain
description The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years.
author Cozzolino,Daniel
Delucchi,Inés
Kholi,Moham
Vázquez,Daniel
author_facet Cozzolino,Daniel
Delucchi,Inés
Kholi,Moham
Vázquez,Daniel
author_sort Cozzolino,Daniel
title Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain
title_short Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain
title_full Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain
title_fullStr Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain
title_full_unstemmed Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain
title_sort use of near infrared reflectance spectroscopy to evaluate quality characteristics in whole-wheat grain
publisher Instituto de Investigaciones Agropecuarias, INIA
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072006000400005
work_keys_str_mv AT cozzolinodaniel useofnearinfraredreflectancespectroscopytoevaluatequalitycharacteristicsinwholewheatgrain
AT delucchiines useofnearinfraredreflectancespectroscopytoevaluatequalitycharacteristicsinwholewheatgrain
AT kholimoham useofnearinfraredreflectancespectroscopytoevaluatequalitycharacteristicsinwholewheatgrain
AT vazquezdaniel useofnearinfraredreflectancespectroscopytoevaluatequalitycharacteristicsinwholewheatgrain
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