Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana

An ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgMand IgA specific antibodies in 45 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences. All th...

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Autores principales:	Contreras,María del C., Acevedo,Elsa, Aguilera,Susana, Sandoval,Lea, Salinas,Patricia
Lenguaje:	Spanish / Castilian
Publicado:	Programa de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de Chile 1999
Materias:	diagnóstico triquinosis ELISA IgM ELISA IgA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94021999000300012
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spelling	oai:scielo:S0365-940219990003000122000-10-04Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humanaContreras,María del C.Acevedo,ElsaAguilera,SusanaSandoval,LeaSalinas,Patricia diagnóstico triquinosis ELISA IgM ELISA IgA An ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgMand IgA specific antibodies in 45 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences. All the patients had positive serology detected by precipitin test, bentonite floculation test, indirect hemagglutination test and ELISA IgG test. The cut-off value was determined using two criteria. Criterion A was determined in each plate, using three positive controls and two negative ones; the average of the negative controls and the weakest positive control, muliplied by a 1.2 factor was, considered the cut-off value. Criterion B was determinated using the average plus three standard deviations from 64 apparently healthy persons serum samples. In both cases, three serum dilutions (1:10, 1:100 and 1:500) were used. The sensitivity of ELISA IgM was 100.0, 93.3 and 82.2% using serum dilutions of 1:10, 1:100 and 1:500 respectively (criterion A) and 100.0, 97.8 and 95.6% for the same dilutions (criterion B), whereas the values for ELISA IgA were: 100.0, 91.1 and 86.7% (criterion A) and 100.0, 100.0 and 91.1%(criterion B). In order to find out the specificity of ELISA IgM and ELISA IgA, additional118 serum samples from individuals with other parasitoses, such as cysticercosis (18) hydatidosis (39), fascioliasis (12), toxocariasis (30), Chagas' disease(12) and individuals with non-specif eosinophilia (7), were also tested. ELISA IgM presented a specificity of 92.3, 93.4 and 97.3% (criterion A) and 96.2, 97.8 and 97.8% (criterion B) whereas the results for ELISA IgA were 97.8, 98.9 and 99.4% (criterion A) and 98.4% for the 1:10 and 1:100 dilutions and 100.0% for the 1:500 dilution (criterion B). The positive predictive values of ELISA IgM were 76.3, 77.8 and 88.1% (criterion A) and 86.5, 91.7 and 91.5% (criterion B) whereas the negative ones were 100.0, 98.3 and 95.7% (criterion A) and 100.0, 99.4 and 98.9% (criterion B). The positive predictive values of ELISA IgA were 91.8, 95.3 and 97.5% (criterion A) and 93.8, 93.8 and 100.0% (criterion B) whereas the negatives ones were: 100.0, 97.8 and 96.8% (criterion A) and 100.0, 100.0 and 97.8%(criterion B). The use of ELISA IgM and ELISA IgA in the immunodiagnosis of trichinosisis discussed.info:eu-repo/semantics/openAccessPrograma de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de ChileBoletín chileno de parasitología v.54 n.3-4 19991999-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94021999000300012es10.4067/S0365-94021999000300012
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	diagnóstico triquinosis ELISA IgM ELISA IgA
spellingShingle	diagnóstico triquinosis ELISA IgM ELISA IgA Contreras,María del C. Acevedo,Elsa Aguilera,Susana Sandoval,Lea Salinas,Patricia Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana
description	An ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgMand IgA specific antibodies in 45 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences. All the patients had positive serology detected by precipitin test, bentonite floculation test, indirect hemagglutination test and ELISA IgG test. The cut-off value was determined using two criteria. Criterion A was determined in each plate, using three positive controls and two negative ones; the average of the negative controls and the weakest positive control, muliplied by a 1.2 factor was, considered the cut-off value. Criterion B was determinated using the average plus three standard deviations from 64 apparently healthy persons serum samples. In both cases, three serum dilutions (1:10, 1:100 and 1:500) were used. The sensitivity of ELISA IgM was 100.0, 93.3 and 82.2% using serum dilutions of 1:10, 1:100 and 1:500 respectively (criterion A) and 100.0, 97.8 and 95.6% for the same dilutions (criterion B), whereas the values for ELISA IgA were: 100.0, 91.1 and 86.7% (criterion A) and 100.0, 100.0 and 91.1%(criterion B). In order to find out the specificity of ELISA IgM and ELISA IgA, additional118 serum samples from individuals with other parasitoses, such as cysticercosis (18) hydatidosis (39), fascioliasis (12), toxocariasis (30), Chagas' disease(12) and individuals with non-specif eosinophilia (7), were also tested. ELISA IgM presented a specificity of 92.3, 93.4 and 97.3% (criterion A) and 96.2, 97.8 and 97.8% (criterion B) whereas the results for ELISA IgA were 97.8, 98.9 and 99.4% (criterion A) and 98.4% for the 1:10 and 1:100 dilutions and 100.0% for the 1:500 dilution (criterion B). The positive predictive values of ELISA IgM were 76.3, 77.8 and 88.1% (criterion A) and 86.5, 91.7 and 91.5% (criterion B) whereas the negative ones were 100.0, 98.3 and 95.7% (criterion A) and 100.0, 99.4 and 98.9% (criterion B). The positive predictive values of ELISA IgA were 91.8, 95.3 and 97.5% (criterion A) and 93.8, 93.8 and 100.0% (criterion B) whereas the negatives ones were: 100.0, 97.8 and 96.8% (criterion A) and 100.0, 100.0 and 97.8%(criterion B). The use of ELISA IgM and ELISA IgA in the immunodiagnosis of trichinosisis discussed.
author	Contreras,María del C. Acevedo,Elsa Aguilera,Susana Sandoval,Lea Salinas,Patricia
author_facet	Contreras,María del C. Acevedo,Elsa Aguilera,Susana Sandoval,Lea Salinas,Patricia
author_sort	Contreras,María del C.
title	Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana
title_short	Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana
title_full	Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana
title_fullStr	Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana
title_full_unstemmed	Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana
title_sort	estandarización de la elisa igm e iga para el inmunodiagnóstico de la triquinosis humana
publisher	Programa de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de Chile
publishDate	1999
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94021999000300012
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Estandarización de la ELISA IgM e IgA para el inmunodiagnóstico de la triquinosis humana

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