Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).

Here, it has been described that normal fresh sera from rainbow trout (Oncorhynchus mykiss) were able to lyse Tylodelphys sp. metacercariae isolated from Galaxias maculatus (Jenyns, 1842) (Teleostei, Galaxiidae) brain. This effect was time dependent, and progressively increased since 1 h up to 18 h...

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Autor principal:	Olabuenaga,Susana
Lenguaje:	Spanish / Castilian
Publicado:	Programa de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de Chile 2000
Materias:	Tylodelphys Galaxias maculatus metacercarias suero trucha Arco Iris complemento
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94022000000100011
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id	oai:scielo:S0365-94022000000100011
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spelling	oai:scielo:S0365-940220000001000112005-07-12Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).Olabuenaga,Susana Tylodelphys Galaxias maculatus metacercarias suero trucha Arco Iris complemento Here, it has been described that normal fresh sera from rainbow trout (Oncorhynchus mykiss) were able to lyse Tylodelphys sp. metacercariae isolated from Galaxias maculatus (Jenyns, 1842) (Teleostei, Galaxiidae) brain. This effect was time dependent, and progressively increased since 1 h up to 18 h incubation. There were oscillations in the ability of different sera samples to lyse these parasites. Similar values were achieved with other salmonid sera, and also with normal human sera pool. The lytic activity was abolished by heat-inactivated sera, by zymosan treatment and by incubation in the presence of EDTA. However, the mean values obtained in the presence of EGTA-Mg++ did not significantly differ from those found with sera as a source of complement. The results of this study suggested the role of complement alternative pathway on Tylodephys sp. in vitro lysis, under our experimental conditions.info:eu-repo/semantics/openAccessPrograma de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de ChileBoletín chileno de parasitología v.55 n.1-2 20002000-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94022000000100011es10.4067/S0365-94022000000100011
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Tylodelphys Galaxias maculatus metacercarias suero trucha Arco Iris complemento
spellingShingle	Tylodelphys Galaxias maculatus metacercarias suero trucha Arco Iris complemento Olabuenaga,Susana Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).
description	Here, it has been described that normal fresh sera from rainbow trout (Oncorhynchus mykiss) were able to lyse Tylodelphys sp. metacercariae isolated from Galaxias maculatus (Jenyns, 1842) (Teleostei, Galaxiidae) brain. This effect was time dependent, and progressively increased since 1 h up to 18 h incubation. There were oscillations in the ability of different sera samples to lyse these parasites. Similar values were achieved with other salmonid sera, and also with normal human sera pool. The lytic activity was abolished by heat-inactivated sera, by zymosan treatment and by incubation in the presence of EDTA. However, the mean values obtained in the presence of EGTA-Mg++ did not significantly differ from those found with sera as a source of complement. The results of this study suggested the role of complement alternative pathway on Tylodephys sp. in vitro lysis, under our experimental conditions.
author	Olabuenaga,Susana
author_facet	Olabuenaga,Susana
author_sort	Olabuenaga,Susana
title	Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).
title_short	Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).
title_full	Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).
title_fullStr	Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).
title_full_unstemmed	Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).
title_sort	efecto in vitro del suero de la trucha arco iris (oncorhynchus mykiss) sobre metacercarias del género tylodelphys (trematoda, diplostomatidae).
publisher	Programa de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de Chile
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94022000000100011
work_keys_str_mv	AT olabuenagasusana efectoinvitrodelsuerodelatruchaarcoirisoncorhynchusmykisssobremetacercariasdelgenerotylodelphystrematodadiplostomatidae
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Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae).

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