Inmunodiagnóstico de la triquinosis humana

An indirect hemagglutination test (IHAT) and an ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgG, IgM and IgA specific antibodies in 113 serum samples from patients confirmed or suspected to have trichinosis by s...

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Autores principales:	Contreras,María del C, Sandoval,Lea, Salinas,Patricia, Saavedra,Tirza, Schenone,Hugo
Lenguaje:	Spanish / Castilian
Publicado:	Programa de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de Chile 2001
Materias:	diagnóstico triquinosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94022001000200003
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spelling	oai:scielo:S0365-940220010002000032009-05-28Inmunodiagnóstico de la triquinosis humanaContreras,María del CSandoval,LeaSalinas,PatriciaSaavedra,TirzaSchenone,Hugo diagnóstico triquinosis An indirect hemagglutination test (IHAT) and an ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgG, IgM and IgA specific antibodies in 113 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences (Group I). The same tests were also performed on 110 serum samples corresponding to patients without strong evidences of having trichinosis (Group II). In Group I the corresponding sensitivities for RHAI, ELISA IgG, ELISA IgM, ELISA IgA were: 82.3-85.8-88.5 and 88.5% respectively. Seventeen patients were tested again a week after the first analysis (10 of them corresponded to negative ones), increasing the positivity: 23.5-100.0;35.3-100.0;41.2-100.0 and 41.2-100.0% for RHAI, ELISA IgG, ELISA IgM and ELISA IgA, respectively. Other two patients were followed-up for 5 years. IHAT and ELISA IgG remained positive, whereas ELISA IgM and ELISA IgA were constantly negative betweeen 17 and 32 months in one case, and between 48 and 60 months in the other (this last one had presented a severe clinic disease). In the group II, four patients were positive with IHAT, however only one for ELISA IgA, the latter also presented ELISA IgM near the cut off. The use of ELISA IgG, ELSIA IgM and ELISA IgA in the immunodiagnosis of trichinosis is discussed.info:eu-repo/semantics/openAccessPrograma de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de ChileBoletín chileno de parasitología v.56 n.3-4 20012001-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94022001000200003es10.4067/S0365-94022001000200003
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	diagnóstico triquinosis
spellingShingle	diagnóstico triquinosis Contreras,María del C Sandoval,Lea Salinas,Patricia Saavedra,Tirza Schenone,Hugo Inmunodiagnóstico de la triquinosis humana
description	An indirect hemagglutination test (IHAT) and an ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgG, IgM and IgA specific antibodies in 113 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences (Group I). The same tests were also performed on 110 serum samples corresponding to patients without strong evidences of having trichinosis (Group II). In Group I the corresponding sensitivities for RHAI, ELISA IgG, ELISA IgM, ELISA IgA were: 82.3-85.8-88.5 and 88.5% respectively. Seventeen patients were tested again a week after the first analysis (10 of them corresponded to negative ones), increasing the positivity: 23.5-100.0;35.3-100.0;41.2-100.0 and 41.2-100.0% for RHAI, ELISA IgG, ELISA IgM and ELISA IgA, respectively. Other two patients were followed-up for 5 years. IHAT and ELISA IgG remained positive, whereas ELISA IgM and ELISA IgA were constantly negative betweeen 17 and 32 months in one case, and between 48 and 60 months in the other (this last one had presented a severe clinic disease). In the group II, four patients were positive with IHAT, however only one for ELISA IgA, the latter also presented ELISA IgM near the cut off. The use of ELISA IgG, ELSIA IgM and ELISA IgA in the immunodiagnosis of trichinosis is discussed.
author	Contreras,María del C Sandoval,Lea Salinas,Patricia Saavedra,Tirza Schenone,Hugo
author_facet	Contreras,María del C Sandoval,Lea Salinas,Patricia Saavedra,Tirza Schenone,Hugo
author_sort	Contreras,María del C
title	Inmunodiagnóstico de la triquinosis humana
title_short	Inmunodiagnóstico de la triquinosis humana
title_full	Inmunodiagnóstico de la triquinosis humana
title_fullStr	Inmunodiagnóstico de la triquinosis humana
title_full_unstemmed	Inmunodiagnóstico de la triquinosis humana
title_sort	inmunodiagnóstico de la triquinosis humana
publisher	Programa de Parasitología. Instituto de Ciencias Biomédicas. Facultad de Medicina. Universidad de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-94022001000200003
work_keys_str_mv	AT contrerasmariadelc inmunodiagnosticodelatriquinosishumana AT sandovallea inmunodiagnosticodelatriquinosishumana AT salinaspatricia inmunodiagnosticodelatriquinosishumana AT saavedratirza inmunodiagnosticodelatriquinosishumana AT schenonehugo inmunodiagnosticodelatriquinosishumana
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Inmunodiagnóstico de la triquinosis humana

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