Cytochrome C reductase immobilized on carbon paste electrode and its electrocatalytic effect on the reduction of cytochrome C

ABSTRACT 	Carbon paste electrodes were modified with cytochrome c reductase enzyme. The behavior of the electrodes was characterized by cyclic voltammetry. The cytochrome c reduction was investigated both on the enzyme modified carbon paste electrode and in a homogeneous system. In the first...

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Autores principales: Merino,M., Nuñez-Vergara,L.J., Squella,J.A.
Lenguaje:English
Publicado: Sociedad Chilena de Química 2000
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0366-16442000000300014
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Sumario:ABSTRACT 	Carbon paste electrodes were modified with cytochrome c reductase enzyme. The behavior of the electrodes was characterized by cyclic voltammetry. The cytochrome c reduction was investigated both on the enzyme modified carbon paste electrode and in a homogeneous system. In the first case, a significant electrocatalytic effect on the reduction of cytochrome c was found. The effect is supported by an increase of 4.3 fold of the peak current and a shift of the cathodic peak potential toward less cathodic values. Also, apparent Michaelis constant values were calculated. 	Results from this work show that carbon paste electrode modified by the cytochrome c reductase represents a new interface that will permit the study of electron transfer reactions involved in biodegradation processes.