SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS

The potential of Capillary Electrophoresis (CE) for separation of C-Glycosylflavonoids has been examined. These compounds are found in Passiflora incarnata L. and Passiflora coerulea L. and their quantitative evaluation is the method for quality control of their extracts. Capillary zone electrophore...

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Autores principales:	Pastene,E. R., Bocaz,G., Peric,I., Montes,M., Silva,V., Riffo,E.
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2000
Materias:	Capillary zone electrophoresis Micellar electrokinetic chromatography C-Glycosylflavonoids Passiflora sp Separation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0366-16442000000300017
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spelling	oai:scielo:S0366-164420000003000172001-03-23SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTSPastene,E. R.Bocaz,G.Peric,I.Montes,M.Silva,V.Riffo,E. Capillary zone electrophoresis Micellar electrokinetic chromatography C-Glycosylflavonoids Passiflora sp Separation The potential of Capillary Electrophoresis (CE) for separation of C-Glycosylflavonoids has been examined. These compounds are found in Passiflora incarnata L. and Passiflora coerulea L. and their quantitative evaluation is the method for quality control of their extracts. Capillary zone electrophoresis (CZE) as well as micellar electrokinetic chromatography (MEKC) conditions were assayed. The optimum resolution of analytes was achieved within 12 min by using an electrolyte system of 50 mM sodium tetraborate, adjusted to pH 9.2 with NaOH and direct detection at 270 nm. Moreover, identification of several peaks was accomplished by repeating and comparing the migration times or electrophoretic mobilities and by spiking the sample solution with the pure compound prior to injectioninfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaBoletín de la Sociedad Chilena de Química v.45 n.3 20002000-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0366-16442000000300017en10.4067/S0366-16442000000300017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Capillary zone electrophoresis Micellar electrokinetic chromatography C-Glycosylflavonoids Passiflora sp Separation
spellingShingle	Capillary zone electrophoresis Micellar electrokinetic chromatography C-Glycosylflavonoids Passiflora sp Separation Pastene,E. R. Bocaz,G. Peric,I. Montes,M. Silva,V. Riffo,E. SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS
description	The potential of Capillary Electrophoresis (CE) for separation of C-Glycosylflavonoids has been examined. These compounds are found in Passiflora incarnata L. and Passiflora coerulea L. and their quantitative evaluation is the method for quality control of their extracts. Capillary zone electrophoresis (CZE) as well as micellar electrokinetic chromatography (MEKC) conditions were assayed. The optimum resolution of analytes was achieved within 12 min by using an electrolyte system of 50 mM sodium tetraborate, adjusted to pH 9.2 with NaOH and direct detection at 270 nm. Moreover, identification of several peaks was accomplished by repeating and comparing the migration times or electrophoretic mobilities and by spiking the sample solution with the pure compound prior to injection
author	Pastene,E. R. Bocaz,G. Peric,I. Montes,M. Silva,V. Riffo,E.
author_facet	Pastene,E. R. Bocaz,G. Peric,I. Montes,M. Silva,V. Riffo,E.
author_sort	Pastene,E. R.
title	SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS
title_short	SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS
title_full	SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS
title_fullStr	SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS
title_full_unstemmed	SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS
title_sort	separation by capillary electrophoresis of c-glycosylflavonoids in passiflora sp. extracts
publisher	Sociedad Chilena de Química
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0366-16442000000300017
work_keys_str_mv	AT pasteneer separationbycapillaryelectrophoresisofcglycosylflavonoidsinpassifloraspextracts AT bocazg separationbycapillaryelectrophoresisofcglycosylflavonoidsinpassifloraspextracts AT perici separationbycapillaryelectrophoresisofcglycosylflavonoidsinpassifloraspextracts AT montesm separationbycapillaryelectrophoresisofcglycosylflavonoidsinpassifloraspextracts AT silvav separationbycapillaryelectrophoresisofcglycosylflavonoidsinpassifloraspextracts AT riffoe separationbycapillaryelectrophoresisofcglycosylflavonoidsinpassifloraspextracts
_version_	1718438115945742336

SEPARATION BY CAPILLARY ELECTROPHORESIS OF C-GLYCOSYLFLAVONOIDS IN Passiflora sp. EXTRACTS

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