LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT

In this study, we have tested the sensitivity of a specific probe such as the polymerase chain reaction (PCR), as an alternative approach to parasitological methods to evaluate patients with cutaneous leishmaniosis (CL). The assay was designed to evaluate familiar group with an infection of 4 months...

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Autores principales:	LUGO DE YARBUH,ANA, PREMOLI-DE-PERCOCO,GLORIA, VALERA,MANUEL
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología 1997
Materias:	Polymerase Chain Reaction Leishmania braziliensis diagnosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300001
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spelling	oai:scielo:S0716-072019970003000012005-09-29LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORTLUGO DE YARBUH,ANAPREMOLI-DE-PERCOCO,GLORIAVALERA,MANUEL Polymerase Chain Reaction Leishmania braziliensis diagnosis In this study, we have tested the sensitivity of a specific probe such as the polymerase chain reaction (PCR), as an alternative approach to parasitological methods to evaluate patients with cutaneous leishmaniosis (CL). The assay was designed to evaluate familiar group with an infection of 4 months. The patients were diagnosed according to clinical criteria, parasitological, immunological assay and PCR specific probe. Five of the family group reported a clinical picture with a lymphadenopathy, localized CL lesions with 2, 15, 27 30 and 60 cutaneous lesions and with different size (mean=5x7 for 27x35mm of diameter), detectable on the exposed body surface: face (cheek, nose, forehead, front), neck, shoulder, hand, forearm and foot. The 6 patients were diagnosed by leishmanin skin test (LST) (mean = 26.2x28.4 mm of diameter). Anti-Leishmania specific antibody were detected by serodiagnosis by the direct agglutination test (DAT),immunofluorescence antibody test (IFAT) and Enzyme-linked immunosorbent assay (ELISA) in all patients. The Leishmania parasite were evident in the lesions of 5 patients, using Giemsa-stained of smear specimens from the lesion, imprint of biopsies lhe lesion, culture and inoculation in hamster of lesions material, sections histological by conventional stained, immunostaining techniques and by PCR assay on serum samples. The PCR procedure increased the sensitivity of direct microscopy diagnostic of CL, therefore should be considered as a valuable and sensitive diagnostic tool in the identification of species of Leishmania and in the diagnosis de CL in the asymptomatic patients.info:eu-repo/semantics/openAccessSociedad Chilena de Parasitología.Federación Latinoamericana de ParasitologíaParasitología al día v.21 n.3-4 19971997-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300001es10.4067/S0716-07201997000300001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Polymerase Chain Reaction Leishmania braziliensis diagnosis
spellingShingle	Polymerase Chain Reaction Leishmania braziliensis diagnosis LUGO DE YARBUH,ANA PREMOLI-DE-PERCOCO,GLORIA VALERA,MANUEL LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT
description	In this study, we have tested the sensitivity of a specific probe such as the polymerase chain reaction (PCR), as an alternative approach to parasitological methods to evaluate patients with cutaneous leishmaniosis (CL). The assay was designed to evaluate familiar group with an infection of 4 months. The patients were diagnosed according to clinical criteria, parasitological, immunological assay and PCR specific probe. Five of the family group reported a clinical picture with a lymphadenopathy, localized CL lesions with 2, 15, 27 30 and 60 cutaneous lesions and with different size (mean=5x7 for 27x35mm of diameter), detectable on the exposed body surface: face (cheek, nose, forehead, front), neck, shoulder, hand, forearm and foot. The 6 patients were diagnosed by leishmanin skin test (LST) (mean = 26.2x28.4 mm of diameter). Anti-Leishmania specific antibody were detected by serodiagnosis by the direct agglutination test (DAT),immunofluorescence antibody test (IFAT) and Enzyme-linked immunosorbent assay (ELISA) in all patients. The Leishmania parasite were evident in the lesions of 5 patients, using Giemsa-stained of smear specimens from the lesion, imprint of biopsies lhe lesion, culture and inoculation in hamster of lesions material, sections histological by conventional stained, immunostaining techniques and by PCR assay on serum samples. The PCR procedure increased the sensitivity of direct microscopy diagnostic of CL, therefore should be considered as a valuable and sensitive diagnostic tool in the identification of species of Leishmania and in the diagnosis de CL in the asymptomatic patients.
author	LUGO DE YARBUH,ANA PREMOLI-DE-PERCOCO,GLORIA VALERA,MANUEL
author_facet	LUGO DE YARBUH,ANA PREMOLI-DE-PERCOCO,GLORIA VALERA,MANUEL
author_sort	LUGO DE YARBUH,ANA
title	LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT
title_short	LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT
title_full	LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT
title_fullStr	LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT
title_full_unstemmed	LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT
title_sort	localized cutaneous leishmaniosis using polymerase chain reaction: a venezuelan family report
publisher	Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología
publishDate	1997
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300001
work_keys_str_mv	AT lugodeyarbuhana localizedcutaneousleishmaniosisusingpolymerasechainreactionavenezuelanfamilyreport AT premolidepercocogloria localizedcutaneousleishmaniosisusingpolymerasechainreactionavenezuelanfamilyreport AT valeramanuel localizedcutaneousleishmaniosisusingpolymerasechainreactionavenezuelanfamilyreport
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LOCALIZED CUTANEOUS LEISHMANIOSIS USING POLYMERASE CHAIN REACTION: A VENEZUELAN FAMILY REPORT

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