MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT

Fasciola hepatica (adult worms)were fixed by the Rossman technique to localize a partially purified proteinase (PPP) from the flukes. Cross and longitudinal sections from paraffine embedded parasites were performed. After microtome sectioning, the slices were incubated with immunized mouse serum or...

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Autores principales: LOPEZ,M, ZERDA,K, BJERCKE,R, NAQUIRA,CESAR, GUERRA,H
Lenguaje:English
Publicado: Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología 1997
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300007
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spelling oai:scielo:S0716-072019970003000072005-09-29MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULTLOPEZ,MZERDA,KBJERCKE,RNAQUIRA,CESARGUERRA,H Fasciola proteinase Immunofluorescence Localization of antigens Fasciola hepatica (adult worms)were fixed by the Rossman technique to localize a partially purified proteinase (PPP) from the flukes. Cross and longitudinal sections from paraffine embedded parasites were performed. After microtome sectioning, the slices were incubated with immunized mouse serum or infected human serum. The following step was staining with fluorescein isothiocyanate (FITC) labelled anti-mouse IgG or anti-human IgG. A positive reaction to the epithelium lining the caecum was observed with the primary antibodiesinfo:eu-repo/semantics/openAccessSociedad Chilena de Parasitología.Federación Latinoamericana de ParasitologíaParasitología al día v.21 n.3-4 19971997-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300007en10.4067/S0716-07201997000300007
institution Scielo Chile
collection Scielo Chile
language English
topic Fasciola proteinase
Immunofluorescence
Localization of antigens
spellingShingle Fasciola proteinase
Immunofluorescence
Localization of antigens
LOPEZ,M
ZERDA,K
BJERCKE,R
NAQUIRA,CESAR
GUERRA,H
MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
description Fasciola hepatica (adult worms)were fixed by the Rossman technique to localize a partially purified proteinase (PPP) from the flukes. Cross and longitudinal sections from paraffine embedded parasites were performed. After microtome sectioning, the slices were incubated with immunized mouse serum or infected human serum. The following step was staining with fluorescein isothiocyanate (FITC) labelled anti-mouse IgG or anti-human IgG. A positive reaction to the epithelium lining the caecum was observed with the primary antibodies
author LOPEZ,M
ZERDA,K
BJERCKE,R
NAQUIRA,CESAR
GUERRA,H
author_facet LOPEZ,M
ZERDA,K
BJERCKE,R
NAQUIRA,CESAR
GUERRA,H
author_sort LOPEZ,M
title MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
title_short MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
title_full MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
title_fullStr MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
title_full_unstemmed MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
title_sort major fasciola hepatica antigens are mainly localized within the digestive tube of the adult
publisher Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología
publishDate 1997
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300007
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