MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT
Fasciola hepatica (adult worms)were fixed by the Rossman technique to localize a partially purified proteinase (PPP) from the flukes. Cross and longitudinal sections from paraffine embedded parasites were performed. After microtome sectioning, the slices were incubated with immunized mouse serum or...
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Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología
1997
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oai:scielo:S0716-072019970003000072005-09-29MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULTLOPEZ,MZERDA,KBJERCKE,RNAQUIRA,CESARGUERRA,H Fasciola proteinase Immunofluorescence Localization of antigens Fasciola hepatica (adult worms)were fixed by the Rossman technique to localize a partially purified proteinase (PPP) from the flukes. Cross and longitudinal sections from paraffine embedded parasites were performed. After microtome sectioning, the slices were incubated with immunized mouse serum or infected human serum. The following step was staining with fluorescein isothiocyanate (FITC) labelled anti-mouse IgG or anti-human IgG. A positive reaction to the epithelium lining the caecum was observed with the primary antibodiesinfo:eu-repo/semantics/openAccessSociedad Chilena de Parasitología.Federación Latinoamericana de ParasitologíaParasitología al día v.21 n.3-4 19971997-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300007en10.4067/S0716-07201997000300007 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
English |
topic |
Fasciola proteinase Immunofluorescence Localization of antigens |
spellingShingle |
Fasciola proteinase Immunofluorescence Localization of antigens LOPEZ,M ZERDA,K BJERCKE,R NAQUIRA,CESAR GUERRA,H MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT |
description |
Fasciola hepatica (adult worms)were fixed by the Rossman technique to localize a partially purified proteinase (PPP) from the flukes. Cross and longitudinal sections from paraffine embedded parasites were performed. After microtome sectioning, the slices were incubated with immunized mouse serum or infected human serum. The following step was staining with fluorescein isothiocyanate (FITC) labelled anti-mouse IgG or anti-human IgG. A positive reaction to the epithelium lining the caecum was observed with the primary antibodies |
author |
LOPEZ,M ZERDA,K BJERCKE,R NAQUIRA,CESAR GUERRA,H |
author_facet |
LOPEZ,M ZERDA,K BJERCKE,R NAQUIRA,CESAR GUERRA,H |
author_sort |
LOPEZ,M |
title |
MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT |
title_short |
MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT |
title_full |
MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT |
title_fullStr |
MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT |
title_full_unstemmed |
MAJOR Fasciola hepatica ANTIGENS ARE MAINLY LOCALIZED WITHIN THE DIGESTIVE TUBE OF THE ADULT |
title_sort |
major fasciola hepatica antigens are mainly localized within the digestive tube of the adult |
publisher |
Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología |
publishDate |
1997 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300007 |
work_keys_str_mv |
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_version_ |
1718439447167500288 |