IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL

The present work had the purpose of detecting the presence of Trichinella spiralis in several tissues and serum in a murine experimental model, during their life cycle. We worked with a lot of 200 female rats Long Evans of 2 and a 1/2 months of age which were infected with 500 infective larvae (IL)...

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Autores principales:	REVELES,R G, VILLALOBOS,R, SALDIVAR,S, MORENO,Mª<FONT FACE=Symbol></FONT>ALEJANDRA
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología 1997
Materias:	Trichinella spiralls Biology Life cycle
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300009
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spelling	oai:scielo:S0716-072019970003000092005-09-29IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTALREVELES,R GVILLALOBOS,RSALDIVAR,SMORENO,Mª<FONT FACE=Symbol></FONT>ALEJANDRA Trichinella spiralls Biology Life cycle The present work had the purpose of detecting the presence of Trichinella spiralis in several tissues and serum in a murine experimental model, during their life cycle. We worked with a lot of 200 female rats Long Evans of 2 and a 1/2 months of age which were infected with 500 infective larvae (IL) approximately. The rats were sacrificed beginning at 24 hours post infection until the day 40, using 5 replicats. Samples of intestinal tissues, heart, diaphragms, masseter tongue and thigh of inferior members were taken every week, the digestion of muscular tissues along with the sampling of serum was carried out each date. The IL obtained at the digestion was processed in paraffin sliced and stained with hematoxiline-eosine. The IL obtained after the digestion, were processed in order to obtain soluble antigen of T. spiralis (AST), sera were analyzed by double immunodiffusion (IDD) and Western blot (WB). lnvasion of skeletal muscle initiates a complex series of changes in both larvae and infected muscle cell, the changes in larvae can be summarized as a growth and maturation of IL. The present sludy contributes of the knowledge of the biology of the parasite during its life cycleinfo:eu-repo/semantics/openAccessSociedad Chilena de Parasitología.Federación Latinoamericana de ParasitologíaParasitología al día v.21 n.3-4 19971997-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300009es10.4067/S0716-07201997000300009
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Trichinella spiralls Biology Life cycle
spellingShingle	Trichinella spiralls Biology Life cycle REVELES,R G VILLALOBOS,R SALDIVAR,S MORENO,Mª<FONT FACE=Symbol></FONT>ALEJANDRA IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL
description	The present work had the purpose of detecting the presence of Trichinella spiralis in several tissues and serum in a murine experimental model, during their life cycle. We worked with a lot of 200 female rats Long Evans of 2 and a 1/2 months of age which were infected with 500 infective larvae (IL) approximately. The rats were sacrificed beginning at 24 hours post infection until the day 40, using 5 replicats. Samples of intestinal tissues, heart, diaphragms, masseter tongue and thigh of inferior members were taken every week, the digestion of muscular tissues along with the sampling of serum was carried out each date. The IL obtained at the digestion was processed in paraffin sliced and stained with hematoxiline-eosine. The IL obtained after the digestion, were processed in order to obtain soluble antigen of T. spiralis (AST), sera were analyzed by double immunodiffusion (IDD) and Western blot (WB). lnvasion of skeletal muscle initiates a complex series of changes in both larvae and infected muscle cell, the changes in larvae can be summarized as a growth and maturation of IL. The present sludy contributes of the knowledge of the biology of the parasite during its life cycle
author	REVELES,R G VILLALOBOS,R SALDIVAR,S MORENO,Mª<FONT FACE=Symbol></FONT>ALEJANDRA
author_facet	REVELES,R G VILLALOBOS,R SALDIVAR,S MORENO,Mª<FONT FACE=Symbol></FONT>ALEJANDRA
author_sort	REVELES,R G
title	IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL
title_short	IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL
title_full	IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL
title_fullStr	IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL
title_full_unstemmed	IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL
title_sort	implante histologico de trichinella spiralis experimental
publisher	Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología
publishDate	1997
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201997000300009
work_keys_str_mv	AT revelesrg implantehistologicodetrichinellaspiralisexperimental AT villalobosr implantehistologicodetrichinellaspiralisexperimental AT saldivars implantehistologicodetrichinellaspiralisexperimental AT morenomafontfacesymbolfontalejandra implantehistologicodetrichinellaspiralisexperimental
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IMPLANTE HISTOLOGICO DE Trichinella spiralis EXPERIMENTAL

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