Hemolytic activity of fresh isolates and clones of Trichomonas gallinae

In in vitro assay the hemolytic activity of live isolates and clones of Trichomonas gallinae was investigated. The fresh isolates and clones were tested against human erythrocytes of groups A, B, AB, and O, and against erythrocytes of six adult animals of different species (rabbit, rat, chicken, hor...

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Autores principales: TASCA,TIANA, DE CARLI,GERALDO A
Lenguaje:English
Publicado: Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología 1999
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201999000300001
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spelling oai:scielo:S0716-072019990003000012000-04-14Hemolytic activity of fresh isolates and clones of Trichomonas gallinaeTASCA,TIANADE CARLI,GERALDO A Trichomonas gallinae hemolytic activity isolates clones In in vitro assay the hemolytic activity of live isolates and clones of Trichomonas gallinae was investigated. The fresh isolates and clones were tested against human erythrocytes of groups A, B, AB, and O, and against erythrocytes of six adult animals of different species (rabbit, rat, chicken, horse, bovine, and sheep). Results showed that each of the isolates and clones tested lysed all human blood groups, as well as rabbit, rat, chicken, horse, bovine and sheep erythrocytes. No hemolysin released by the parasites could be identified. Parasite culture supernatants of hemolysis assays, tested in the presence of human erythrocytes did not induce any hemolytic activity. Trichomonads culture supernatants from 18, 24, and 48 hr extracts of sonicated parasites or cold killed organisms tested with human erythrocytes did not exhibit hemolysis. Although an hemolytic activity of T. gallinae was clearly demonstrated, a relationship between hemolysis and cell pathogenicity is not yet clearly established.info:eu-repo/semantics/openAccessSociedad Chilena de Parasitología.Federación Latinoamericana de ParasitologíaParasitología al día v.23 n.3-4 19991999-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201999000300001en10.4067/S0716-07201999000300001
institution Scielo Chile
collection Scielo Chile
language English
topic Trichomonas gallinae
hemolytic activity
isolates
clones
spellingShingle Trichomonas gallinae
hemolytic activity
isolates
clones
TASCA,TIANA
DE CARLI,GERALDO A
Hemolytic activity of fresh isolates and clones of Trichomonas gallinae
description In in vitro assay the hemolytic activity of live isolates and clones of Trichomonas gallinae was investigated. The fresh isolates and clones were tested against human erythrocytes of groups A, B, AB, and O, and against erythrocytes of six adult animals of different species (rabbit, rat, chicken, horse, bovine, and sheep). Results showed that each of the isolates and clones tested lysed all human blood groups, as well as rabbit, rat, chicken, horse, bovine and sheep erythrocytes. No hemolysin released by the parasites could be identified. Parasite culture supernatants of hemolysis assays, tested in the presence of human erythrocytes did not induce any hemolytic activity. Trichomonads culture supernatants from 18, 24, and 48 hr extracts of sonicated parasites or cold killed organisms tested with human erythrocytes did not exhibit hemolysis. Although an hemolytic activity of T. gallinae was clearly demonstrated, a relationship between hemolysis and cell pathogenicity is not yet clearly established.
author TASCA,TIANA
DE CARLI,GERALDO A
author_facet TASCA,TIANA
DE CARLI,GERALDO A
author_sort TASCA,TIANA
title Hemolytic activity of fresh isolates and clones of Trichomonas gallinae
title_short Hemolytic activity of fresh isolates and clones of Trichomonas gallinae
title_full Hemolytic activity of fresh isolates and clones of Trichomonas gallinae
title_fullStr Hemolytic activity of fresh isolates and clones of Trichomonas gallinae
title_full_unstemmed Hemolytic activity of fresh isolates and clones of Trichomonas gallinae
title_sort hemolytic activity of fresh isolates and clones of trichomonas gallinae
publisher Sociedad Chilena de Parasitología.Federación Latinoamericana de Parasitología
publishDate 1999
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07201999000300001
work_keys_str_mv AT tascatiana hemolyticactivityoffreshisolatesandclonesoftrichomonasgallinae
AT decarligeraldoa hemolyticactivityoffreshisolatesandclonesoftrichomonasgallinae
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