Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine

Mouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the ran...

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Autores principales:	LÓPEZ-SOLÍS,REMIGIO, PUENTE,MARGARITA, DURÁN,VIVIANA, MORALES-BOZO,IRENE, KEMMERLING,ULRIKE, PARDO,RODRIGO, WENK,CAROLINA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	mouse saliva secretion pilocarpine polypeptides
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023
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spelling	oai:scielo:S0716-078X20010000000232001-06-27Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpineLÓPEZ-SOLÍS,REMIGIOPUENTE,MARGARITADURÁN,VIVIANAMORALES-BOZO,IRENEKEMMERLING,ULRIKEPARDO,RODRIGOWENK,CAROLINA mouse saliva secretion pilocarpine polypeptides Mouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the range 40-400 µg provoked a dose-dependent secretory response. The secretory response lasted for about 40 minutes and was constituted by four clearly defined stages, namely: lag (5-10 min), maximal flow (10 min), slowering (15-20 min) and postsecretory rest. In this response, usual parameters were: maximal flow rate, 30-40 µl/min; total volume of saliva, 250-350 µl/mouse and total salivary protein, about 700 µg/mouse. Temporal desensitization of the secretory response was observed within the first hour following a single stimulation by pilocarpine. However, recurrent stimulations by this agonist given at 24-h intervals produced an equally intense secretory response, thus suggesting resensitization during that period. The polypeptide composition of salivas obtained from a number of mice after a first pilocarpine stimulation were undistinguishable from each other. That salivary polypeptide pattern was also observed in a series of salivas obtained day by day from single animals stimulated at 24-h intervals by the agonist. Thus, both the characteristics of the secretory response as well as the polypeptide composition of mouse saliva after short-term or long-term challenges by pilocarpine were found to be highly consistent. Accordingly, these studies open the possibility of accomplishing a systematic molecular typing of saliva from individual living mice either from natural populations or from mice subjected to experimental laboratory conditionsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileRevista chilena de historia natural v.74 n.1 20012001-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023en10.4067/S0716-078X2001000000023
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	mouse saliva secretion pilocarpine polypeptides
spellingShingle	mouse saliva secretion pilocarpine polypeptides LÓPEZ-SOLÍS,REMIGIO PUENTE,MARGARITA DURÁN,VIVIANA MORALES-BOZO,IRENE KEMMERLING,ULRIKE PARDO,RODRIGO WENK,CAROLINA Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
description	Mouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the range 40-400 µg provoked a dose-dependent secretory response. The secretory response lasted for about 40 minutes and was constituted by four clearly defined stages, namely: lag (5-10 min), maximal flow (10 min), slowering (15-20 min) and postsecretory rest. In this response, usual parameters were: maximal flow rate, 30-40 µl/min; total volume of saliva, 250-350 µl/mouse and total salivary protein, about 700 µg/mouse. Temporal desensitization of the secretory response was observed within the first hour following a single stimulation by pilocarpine. However, recurrent stimulations by this agonist given at 24-h intervals produced an equally intense secretory response, thus suggesting resensitization during that period. The polypeptide composition of salivas obtained from a number of mice after a first pilocarpine stimulation were undistinguishable from each other. That salivary polypeptide pattern was also observed in a series of salivas obtained day by day from single animals stimulated at 24-h intervals by the agonist. Thus, both the characteristics of the secretory response as well as the polypeptide composition of mouse saliva after short-term or long-term challenges by pilocarpine were found to be highly consistent. Accordingly, these studies open the possibility of accomplishing a systematic molecular typing of saliva from individual living mice either from natural populations or from mice subjected to experimental laboratory conditions
author	LÓPEZ-SOLÍS,REMIGIO PUENTE,MARGARITA DURÁN,VIVIANA MORALES-BOZO,IRENE KEMMERLING,ULRIKE PARDO,RODRIGO WENK,CAROLINA
author_facet	LÓPEZ-SOLÍS,REMIGIO PUENTE,MARGARITA DURÁN,VIVIANA MORALES-BOZO,IRENE KEMMERLING,ULRIKE PARDO,RODRIGO WENK,CAROLINA
author_sort	LÓPEZ-SOLÍS,REMIGIO
title	Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
title_short	Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
title_full	Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
title_fullStr	Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
title_full_unstemmed	Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
title_sort	characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023
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Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine

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