Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation

The assessment of the effects of Ocean Acidification (OA) on marine life has received increasing attention in recent marine research. On a mesocosmic scale, the CO2 levels in seawater can be manipulated to evaluate experimentally the consequences of OA on marine organisms (vertebrates and invertebra...

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Autores principales:	TORRES,RODRIGO, MANRIQUEZ,PATRICIO H, DUARTE,CRISTIAN, NAVARRO,JORGE M, LAGOS,NELSON A, VARGAS,CRISTIAN A, LARDIES,MARCO A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2013
Materias:	carbonate chemistry manipulation ocean acidification
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2013000400006
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spelling	oai:scielo:S0716-078X20130004000062014-02-03Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulationTORRES,RODRIGOMANRIQUEZ,PATRICIO HDUARTE,CRISTIANNAVARRO,JORGE MLAGOS,NELSON AVARGAS,CRISTIAN ALARDIES,MARCO A carbonate chemistry manipulation ocean acidification The assessment of the effects of Ocean Acidification (OA) on marine life has received increasing attention in recent marine research. On a mesocosmic scale, the CO2 levels in seawater can be manipulated to evaluate experimentally the consequences of OA on marine organisms (vertebrates and invertebrates). An ideal manipulation of carbonate chemistry should mimic exactly the changes to carbonate chemistry, which will occur in years to come. Although some methods have been described in the literature, here we describe in detail a simple, robust and inexpensive system to produce CO2-enriched seawater by bubbling the seawater with air-CO2 mixtures. The system uses mass flow controllers (MFC) to blend atmospheric air with pure CO2 to produce two pCO2 levels. The air-CO2 mixtures are delivered continuously to seawater equilibration reservoirs, and simultaneously to an infrared CO2 analyser to verify CO2 levels in the air-CO2 mixture delivered to the equilibration tanks. We monitored both pH and total alkalinity in the equilibration reservoirs over a period of one year in order to document the long-term performance of this system for simulating the future carbonate chemistry of seawater in a coastal laboratory. System performance was sufficient to maintain three contrasting (e.g., 397, 709 and 1032 matm) and relatively constant (the coefficient of variability was 11 %, 9 % and 9 % respectively) seawater pCO2 during a year-long monitoring.info:eu-repo/semantics/openAccessSociedad de Biología de ChileRevista chilena de historia natural v.86 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2013000400006en10.4067/S0716-078X2013000400006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	carbonate chemistry manipulation ocean acidification
spellingShingle	carbonate chemistry manipulation ocean acidification TORRES,RODRIGO MANRIQUEZ,PATRICIO H DUARTE,CRISTIAN NAVARRO,JORGE M LAGOS,NELSON A VARGAS,CRISTIAN A LARDIES,MARCO A Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
description	The assessment of the effects of Ocean Acidification (OA) on marine life has received increasing attention in recent marine research. On a mesocosmic scale, the CO2 levels in seawater can be manipulated to evaluate experimentally the consequences of OA on marine organisms (vertebrates and invertebrates). An ideal manipulation of carbonate chemistry should mimic exactly the changes to carbonate chemistry, which will occur in years to come. Although some methods have been described in the literature, here we describe in detail a simple, robust and inexpensive system to produce CO2-enriched seawater by bubbling the seawater with air-CO2 mixtures. The system uses mass flow controllers (MFC) to blend atmospheric air with pure CO2 to produce two pCO2 levels. The air-CO2 mixtures are delivered continuously to seawater equilibration reservoirs, and simultaneously to an infrared CO2 analyser to verify CO2 levels in the air-CO2 mixture delivered to the equilibration tanks. We monitored both pH and total alkalinity in the equilibration reservoirs over a period of one year in order to document the long-term performance of this system for simulating the future carbonate chemistry of seawater in a coastal laboratory. System performance was sufficient to maintain three contrasting (e.g., 397, 709 and 1032 matm) and relatively constant (the coefficient of variability was 11 %, 9 % and 9 % respectively) seawater pCO2 during a year-long monitoring.
author	TORRES,RODRIGO MANRIQUEZ,PATRICIO H DUARTE,CRISTIAN NAVARRO,JORGE M LAGOS,NELSON A VARGAS,CRISTIAN A LARDIES,MARCO A
author_facet	TORRES,RODRIGO MANRIQUEZ,PATRICIO H DUARTE,CRISTIAN NAVARRO,JORGE M LAGOS,NELSON A VARGAS,CRISTIAN A LARDIES,MARCO A
author_sort	TORRES,RODRIGO
title	Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
title_short	Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
title_full	Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
title_fullStr	Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
title_full_unstemmed	Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
title_sort	evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation
publisher	Sociedad de Biología de Chile
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2013000400006
work_keys_str_mv	AT torresrodrigo evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation AT manriquezpatricioh evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation AT duartecristian evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation AT navarrojorgem evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation AT lagosnelsona evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation AT vargascristiana evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation AT lardiesmarcoa evaluationofasemiautomaticsystemforlongtermseawatercarbonatechemistrymanipulation
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Evaluation of a semi-automatic system for long-term seawater carbonate chemistry manipulation

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