Rojo neutro: Nuestra experiencia de diagnóstico en micosis superficiales en cinco pacientes

Due to differences in the result of the Direct Microscopic Test (DMT) and culture for fungi, the technique of neutral red supravital coloration has been put to practice and in this manner, evaluate the mycological cure. Patients and Methods: In order to achieve this purpose, 30 patients with presump...

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Autores principales: Carballo,Graciela M., Ambasch,Germán, Peralta,Nora B., García,Silvina
Lenguaje:Spanish / Castilian
Publicado: Sociedad Chilena de Infectología 2002
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182002000400008
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Sumario:Due to differences in the result of the Direct Microscopic Test (DMT) and culture for fungi, the technique of neutral red supravital coloration has been put to practice and in this manner, evaluate the mycological cure. Patients and Methods: In order to achieve this purpose, 30 patients with presumptive diagnosis of superficial mycosis submitted from the Capital City of Córdoba and surrounding districts were evaluated. Once the laboratory diagnosis was confirmed, clinical cases with the following requirements were selected: patients with skin mycosis in different zones; had not received previous treatment for mycosis. Five patients who fulfilled both previous requirements, wanted to receive complete antifungal treatment and accepted to undergo both, clinical and laboratory periodical follow _ups, were enrolled. The material obtained from the selected patients was assessed with the habitual technique of microscopic observation (KOH and heat). At the same time fungal cultures were performed, the coloration of neutral red was utilized in each of the selected patients. The results obtained were very promising. This fact allowed us to put this technique into practice as a routine. Besides, its low price constitutes an advantage that can be applied to any laboratory that makes mycologic diagnosis