Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos

The diagnostic applications of the molecular biology in infectious diseases are wide and applicable to any diagnostic problem. In the Herpesviridae family, the most used methods are those based on the amplification of DNA polymerase gene for the detection of HSV 1 and 2, varicela-zoster, citomegalov...

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Autores principales:	Corvalán R.,Alejandro, Aguayo G.,Francisco, Lévican G.,Jorge, Corvalán V.,Ignacio
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Chilena de Infectología 2003
Materias:	Infectious disease Molecular biology Molecular diagnosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182003000100004
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spelling	oai:scielo:S0716-101820030001000042003-06-04Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciososCorvalán R.,AlejandroAguayo G.,FranciscoLévican G.,JorgeCorvalán V.,Ignacio Infectious disease Molecular biology Molecular diagnosis The diagnostic applications of the molecular biology in infectious diseases are wide and applicable to any diagnostic problem. In the Herpesviridae family, the most used methods are those based on the amplification of DNA polymerase gene for the detection of HSV 1 and 2, varicela-zoster, citomegalovirus, Epstein Barr virus and HHV6 simultaneously. This methodology has been able of detect the co-infection of HSV1 and VZV in samples of CNS fluid. In citomegalovirus, molecular methods are used in the monitoring of the reactivation of CMV in immunosuppressed patients and are able to detect viral reactivation within 1 week before symptoms. The molecular methods are also able to identify the Epstein-Barr virus in a proportion of 8 to 20% of gastric cancer cases harboring a unique strain in spite of the presence multiples strains in the healthy population. These associations between virus and cancer have also been described for the human papilloma virus and esophageal and lung cancer. In bacterial agents, the detection and quantification of Bordetella pertussis is another interesting application since it might become a method for rapid diagnosis and predictive of severity in children less than 6 months old. The identification of Helicobacter pylori strains in relation to gastric cancer and peptic ulcer disease and the characterization of strains of methicillin resistant Staphylococcus aureus are other examples of potential applications of the molecular methods in typing microorganisms. In the diagnosis of respiratory tract infectious agents such as Mycobacterium tuberculosis, Pneumocystis carinii and atypical agents, the molecular methods allow the diagnosis in non-invasive samples. Finally, these new methodologies also contribute to the diagnosis of systemic mycotic agents (Candidiasis and Aspergillosis) particularly in immunosuppressed patientsinfo:eu-repo/semantics/openAccessSociedad Chilena de InfectologíaRevista chilena de infectología v.20 n.1 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182003000100004es10.4067/S0716-10182003000100004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Infectious disease Molecular biology Molecular diagnosis
spellingShingle	Infectious disease Molecular biology Molecular diagnosis Corvalán R.,Alejandro Aguayo G.,Francisco Lévican G.,Jorge Corvalán V.,Ignacio Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos
description	The diagnostic applications of the molecular biology in infectious diseases are wide and applicable to any diagnostic problem. In the Herpesviridae family, the most used methods are those based on the amplification of DNA polymerase gene for the detection of HSV 1 and 2, varicela-zoster, citomegalovirus, Epstein Barr virus and HHV6 simultaneously. This methodology has been able of detect the co-infection of HSV1 and VZV in samples of CNS fluid. In citomegalovirus, molecular methods are used in the monitoring of the reactivation of CMV in immunosuppressed patients and are able to detect viral reactivation within 1 week before symptoms. The molecular methods are also able to identify the Epstein-Barr virus in a proportion of 8 to 20% of gastric cancer cases harboring a unique strain in spite of the presence multiples strains in the healthy population. These associations between virus and cancer have also been described for the human papilloma virus and esophageal and lung cancer. In bacterial agents, the detection and quantification of Bordetella pertussis is another interesting application since it might become a method for rapid diagnosis and predictive of severity in children less than 6 months old. The identification of Helicobacter pylori strains in relation to gastric cancer and peptic ulcer disease and the characterization of strains of methicillin resistant Staphylococcus aureus are other examples of potential applications of the molecular methods in typing microorganisms. In the diagnosis of respiratory tract infectious agents such as Mycobacterium tuberculosis, Pneumocystis carinii and atypical agents, the molecular methods allow the diagnosis in non-invasive samples. Finally, these new methodologies also contribute to the diagnosis of systemic mycotic agents (Candidiasis and Aspergillosis) particularly in immunosuppressed patients
author	Corvalán R.,Alejandro Aguayo G.,Francisco Lévican G.,Jorge Corvalán V.,Ignacio
author_facet	Corvalán R.,Alejandro Aguayo G.,Francisco Lévican G.,Jorge Corvalán V.,Ignacio
author_sort	Corvalán R.,Alejandro
title	Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos
title_short	Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos
title_full	Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos
title_fullStr	Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos
title_full_unstemmed	Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos
title_sort	biología molecular en infectología: parte ii: diagnóstico molecular de agentes infecciosos
publisher	Sociedad Chilena de Infectología
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182003000100004
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Biología molecular en Infectología: Parte II: Diagnóstico molecular de agentes infecciosos

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