Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing

Abstract The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has...

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Autores principales:	Farfan,Mauricio J., Torres,Juan P., O'Ryan,Miguel, Olivares,Mauricio, Gallardo,Pablo, Lastra,Jorge, Salas,Carolina
Lenguaje:	English
Publicado:	Sociedad Chilena de Infectología 2020
Materias:	coronavirus SARS-CoV-2 COVID-19 diagnosis pool testing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182020000300276
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spelling	oai:scielo:S0716-101820200003002762020-08-06Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testingFarfan,Mauricio J.Torres,Juan P.O'Ryan,MiguelOlivares,MauricioGallardo,PabloLastra,JorgeSalas,Carolina coronavirus SARS-CoV-2 COVID-19 diagnosis pool testing Abstract The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, we found that pooling of 5 samples showed that CT variations were in the range of 1.0-4,5 units, with less likelihood of a false negative result. Results of the sample without nucleic acid ex-traction, was unsatisfactory, with a significant increase in CT values, and thus for risk of a false negative result. In conclusion, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.info:eu-repo/semantics/openAccessSociedad Chilena de InfectologíaRevista chilena de infectología v.37 n.3 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182020000300276en10.4067/s0716-10182020000300276
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	coronavirus SARS-CoV-2 COVID-19 diagnosis pool testing
spellingShingle	coronavirus SARS-CoV-2 COVID-19 diagnosis pool testing Farfan,Mauricio J. Torres,Juan P. O'Ryan,Miguel Olivares,Mauricio Gallardo,Pablo Lastra,Jorge Salas,Carolina Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
description	Abstract The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, we found that pooling of 5 samples showed that CT variations were in the range of 1.0-4,5 units, with less likelihood of a false negative result. Results of the sample without nucleic acid ex-traction, was unsatisfactory, with a significant increase in CT values, and thus for risk of a false negative result. In conclusion, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.
author	Farfan,Mauricio J. Torres,Juan P. O'Ryan,Miguel Olivares,Mauricio Gallardo,Pablo Lastra,Jorge Salas,Carolina
author_facet	Farfan,Mauricio J. Torres,Juan P. O'Ryan,Miguel Olivares,Mauricio Gallardo,Pablo Lastra,Jorge Salas,Carolina
author_sort	Farfan,Mauricio J.
title	Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
title_short	Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
title_full	Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
title_fullStr	Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
title_full_unstemmed	Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
title_sort	optimizing rt-pcr detection of sars-cov-2 for developing countries using pool testing
publisher	Sociedad Chilena de Infectología
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182020000300276
work_keys_str_mv	AT farfanmauricioj optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting AT torresjuanp optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting AT oryanmiguel optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting AT olivaresmauricio optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting AT gallardopablo optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting AT lastrajorge optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting AT salascarolina optimizingrtpcrdetectionofsarscov2fordevelopingcountriesusingpooltesting
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Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing

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