Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat

Taiep is an autosomal recessive mutant rat that shows a highly hypomyelinated central nervous system (CNS). Oligodendrocytes accumulate microtubules (MTs) in association with endoplasmic reticulum (ER) membranes forming MT-ER complexes. The microtubular defect in oligodendrocytes, the abnormal forma...

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Autores principales:	KRSULOVIC,JUAN, COUVE,EDUARDO, RONCAGLIOLO,MANUEL
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 1999
Materias:	myelin mutant taiep rat optic nerve dysmyelination demyelination astrogliosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400005
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spelling	oai:scielo:S0716-976019990004000052000-11-27Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep ratKRSULOVIC,JUANCOUVE,EDUARDORONCAGLIOLO,MANUEL myelin mutant taiep rat optic nerve dysmyelination demyelination astrogliosis Taiep is an autosomal recessive mutant rat that shows a highly hypomyelinated central nervous system (CNS). Oligodendrocytes accumulate microtubules (MTs) in association with endoplasmic reticulum (ER) membranes forming MT-ER complexes. The microtubular defect in oligodendrocytes, the abnormal formation of CNS myelin and the astrocytic reaction were characterized by immunocytochemical and ultrastructural methods during the first year of life. Optic nerves of both control and taiep rats were processed by the immunoperoxidase method using antibodies against tubulin, myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP). Taiep oligodendrocytes are strongly immunoreactive against tubulin, indicative of a significant accumulation of microtubules. Early differentiated oligodendrocytes observed with electron microscopy show that MT-ER complexes are mainly present in the cell body. This defect increases during the first year of life; oligodendrocytes show large MT-ER complexes projected within oligodendrocyte processes. Using anti-MBP, there was a progressive reduction of immunolabeling in the myelin sheaths as taiep rats grew older. Ultrastructural analysis revealed severely dysmyelinated axons with a frequently collapsed periaxonal collar. However, through age the myelin sheath became gradually infiltrated by MTs, suggesting their contribution to premature loss of myelin in the taiep rat. Axons of one-year-old taiep rats were severely demyelinated. Modifications in astrocytes revealed by the GFAP antibody showed a strong hypertrophy with increased immunostaining in their processes. As demyelination of axons progressed, taiep rats developed a strong astrogliosis. The present findings suggest that in taiep rats the early abnormal myelination of axons affects the adequate maintenance of myelin, leading to a progressive loss of myelin components and severe astrogliosis, features that should be considered in the pathogenesis of dysmyelinating diseasesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.32 n.4 19991999-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400005en10.4067/S0716-97601999000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	myelin mutant taiep rat optic nerve dysmyelination demyelination astrogliosis
spellingShingle	myelin mutant taiep rat optic nerve dysmyelination demyelination astrogliosis KRSULOVIC,JUAN COUVE,EDUARDO RONCAGLIOLO,MANUEL Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
description	Taiep is an autosomal recessive mutant rat that shows a highly hypomyelinated central nervous system (CNS). Oligodendrocytes accumulate microtubules (MTs) in association with endoplasmic reticulum (ER) membranes forming MT-ER complexes. The microtubular defect in oligodendrocytes, the abnormal formation of CNS myelin and the astrocytic reaction were characterized by immunocytochemical and ultrastructural methods during the first year of life. Optic nerves of both control and taiep rats were processed by the immunoperoxidase method using antibodies against tubulin, myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP). Taiep oligodendrocytes are strongly immunoreactive against tubulin, indicative of a significant accumulation of microtubules. Early differentiated oligodendrocytes observed with electron microscopy show that MT-ER complexes are mainly present in the cell body. This defect increases during the first year of life; oligodendrocytes show large MT-ER complexes projected within oligodendrocyte processes. Using anti-MBP, there was a progressive reduction of immunolabeling in the myelin sheaths as taiep rats grew older. Ultrastructural analysis revealed severely dysmyelinated axons with a frequently collapsed periaxonal collar. However, through age the myelin sheath became gradually infiltrated by MTs, suggesting their contribution to premature loss of myelin in the taiep rat. Axons of one-year-old taiep rats were severely demyelinated. Modifications in astrocytes revealed by the GFAP antibody showed a strong hypertrophy with increased immunostaining in their processes. As demyelination of axons progressed, taiep rats developed a strong astrogliosis. The present findings suggest that in taiep rats the early abnormal myelination of axons affects the adequate maintenance of myelin, leading to a progressive loss of myelin components and severe astrogliosis, features that should be considered in the pathogenesis of dysmyelinating diseases
author	KRSULOVIC,JUAN COUVE,EDUARDO RONCAGLIOLO,MANUEL
author_facet	KRSULOVIC,JUAN COUVE,EDUARDO RONCAGLIOLO,MANUEL
author_sort	KRSULOVIC,JUAN
title	Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
title_short	Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
title_full	Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
title_fullStr	Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
title_full_unstemmed	Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
title_sort	dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
publisher	Sociedad de Biología de Chile
publishDate	1999
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400005
work_keys_str_mv	AT krsulovicjuan dysmyelinationdemyelinationandreactiveastrogliosisintheopticnerveofthetaieprat AT couveeduardo dysmyelinationdemyelinationandreactiveastrogliosisintheopticnerveofthetaieprat AT roncagliolomanuel dysmyelinationdemyelinationandreactiveastrogliosisintheopticnerveofthetaieprat
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Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat

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