Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals

The antioxidant ability of nitric oxide (NO) generated by a chemical donor and of commercially available antioxidant preparations was assayed. SNAP (S-Nitroso-N-acetylpenicilamine) was used as the NO donor, and Ginkgo biloba, wheat and alfalfa preparations were tested. Lipid peroxidation was assayed...

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Autores principales:	BOVERIS,ALEJANDRO D, GALATRO,ANDREA, PUNTARULO,SUSANA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2000
Materias:	antioxidants nitric oxide plants extracts lipid radicals
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200016
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spelling	oai:scielo:S0716-976020000002000162010-02-11Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicalsBOVERIS,ALEJANDRO DGALATRO,ANDREAPUNTARULO,SUSANA antioxidants nitric oxide plants extracts lipid radicals The antioxidant ability of nitric oxide (NO) generated by a chemical donor and of commercially available antioxidant preparations was assayed. SNAP (S-Nitroso-N-acetylpenicilamine) was used as the NO donor, and Ginkgo biloba, wheat and alfalfa preparations were tested. Lipid peroxidation was assayed by EPR employing a reaction system consisting of rat liver microsomes, ADP, FeCl3, NADPH and POBN in phosphate buffer, pH=7.4. In vitro NO exposure decreased microsomal lipid peroxidation in a dose-dependent manner. The dose responsible for inhibiting the microsomal content of lipid radical adducts by 50% (LD50) for SNAP was 550 mu M (NO generation rate 0.1 mu M/min). The addition of 50 mM hemoglobin to the incubation media prevented NO effect on lipid peroxidation. The addition of an amount of the antioxidant preparations equivalent to the LD50 doses inhibited lipid peroxidation by 21, 15, and 33% for wheat, alfalfa, Ginkgo biloba preparations respectively in the presence of 550 mu M SNAP. We detected a decrease in the content of lipid radical adducts after simultaneous supplementation, although it was less than 50%, even when LD50 doses of the products were added. This suggests that NO and the natural antioxidants inhibit lipid peroxidation by a mechanism that has both common and non-shared featuresinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.33 n.2 20002000-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200016en10.4067/S0716-97602000000200016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antioxidants nitric oxide plants extracts lipid radicals
spellingShingle	antioxidants nitric oxide plants extracts lipid radicals BOVERIS,ALEJANDRO D GALATRO,ANDREA PUNTARULO,SUSANA Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
description	The antioxidant ability of nitric oxide (NO) generated by a chemical donor and of commercially available antioxidant preparations was assayed. SNAP (S-Nitroso-N-acetylpenicilamine) was used as the NO donor, and Ginkgo biloba, wheat and alfalfa preparations were tested. Lipid peroxidation was assayed by EPR employing a reaction system consisting of rat liver microsomes, ADP, FeCl3, NADPH and POBN in phosphate buffer, pH=7.4. In vitro NO exposure decreased microsomal lipid peroxidation in a dose-dependent manner. The dose responsible for inhibiting the microsomal content of lipid radical adducts by 50% (LD50) for SNAP was 550 mu M (NO generation rate 0.1 mu M/min). The addition of 50 mM hemoglobin to the incubation media prevented NO effect on lipid peroxidation. The addition of an amount of the antioxidant preparations equivalent to the LD50 doses inhibited lipid peroxidation by 21, 15, and 33% for wheat, alfalfa, Ginkgo biloba preparations respectively in the presence of 550 mu M SNAP. We detected a decrease in the content of lipid radical adducts after simultaneous supplementation, although it was less than 50%, even when LD50 doses of the products were added. This suggests that NO and the natural antioxidants inhibit lipid peroxidation by a mechanism that has both common and non-shared features
author	BOVERIS,ALEJANDRO D GALATRO,ANDREA PUNTARULO,SUSANA
author_facet	BOVERIS,ALEJANDRO D GALATRO,ANDREA PUNTARULO,SUSANA
author_sort	BOVERIS,ALEJANDRO D
title	Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
title_short	Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
title_full	Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
title_fullStr	Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
title_full_unstemmed	Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
title_sort	effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
publisher	Sociedad de Biología de Chile
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200016
work_keys_str_mv	AT boverisalejandrod effectofnitricoxideandplantantioxidantsonmicrosomalcontentoflipidradicals AT galatroandrea effectofnitricoxideandplantantioxidantsonmicrosomalcontentoflipidradicals AT puntarulosusana effectofnitricoxideandplantantioxidantsonmicrosomalcontentoflipidradicals
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Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals

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