Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos

Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such s...

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Autores principales:	GHATPANDE,SATISH, SHAFIQ,SAIYID, SIDDIQUI,M.A.Q.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	Cardiogenesis cardiomyopathy myosin light chains tissue culture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009
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spelling	oai:scielo:S0716-976020010001000092001-09-27Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken EmbryosGHATPANDE,SATISHSHAFIQ,SAIYIDSIDDIQUI,M.A.Q. Cardiogenesis cardiomyopathy myosin light chains tissue culture Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe deficiency in formation of striated myofibrils. Further studies with antisense mRNA techniques of whole embryo cultures should, therefore, be useful to evaluate the role of vMLC2 and other putative regulatory factors in cardiac myofibrillogenesisinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.1 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009en10.4067/S0716-97602001000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cardiogenesis cardiomyopathy myosin light chains tissue culture
spellingShingle	Cardiogenesis cardiomyopathy myosin light chains tissue culture GHATPANDE,SATISH SHAFIQ,SAIYID SIDDIQUI,M.A.Q. Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
description	Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe deficiency in formation of striated myofibrils. Further studies with antisense mRNA techniques of whole embryo cultures should, therefore, be useful to evaluate the role of vMLC2 and other putative regulatory factors in cardiac myofibrillogenesis
author	GHATPANDE,SATISH SHAFIQ,SAIYID SIDDIQUI,M.A.Q.
author_facet	GHATPANDE,SATISH SHAFIQ,SAIYID SIDDIQUI,M.A.Q.
author_sort	GHATPANDE,SATISH
title	Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
title_short	Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
title_full	Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
title_fullStr	Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
title_full_unstemmed	Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
title_sort	ventricular myosin light chain-2 gene expression in developing heart of chicken embryos
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009
work_keys_str_mv	AT ghatpandesatish ventricularmyosinlightchain2geneexpressionindevelopingheartofchickenembryos AT shafiqsaiyid ventricularmyosinlightchain2geneexpressionindevelopingheartofchickenembryos AT siddiquimaq ventricularmyosinlightchain2geneexpressionindevelopingheartofchickenembryos
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Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos

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