In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)

We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. W...

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Autores principales:	LÓPEZ,MAURICIO, FIGUEROA,JAIME, KAUSEL,GUDRUN, VERA,MARÍA INÉS, KRAUSKOPF,MANUEL
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	Acclimatization In situ hybridization Somatolactin expression Pituitary Carp Fish
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010
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spelling	oai:scielo:S0716-976020010001000102001-09-27In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)LÓPEZ,MAURICIOFIGUEROA,JAIMEKAUSEL,GUDRUNVERA,MARÍA INÉSKRAUSKOPF,MANUEL Acclimatization In situ hybridization Somatolactin expression Pituitary Carp Fish We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the regulation of the somatolactin detected with the probe used at the transcriptional level in pituitary glandsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.1 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010en10.4067/S0716-97602001000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Acclimatization In situ hybridization Somatolactin expression Pituitary Carp Fish
spellingShingle	Acclimatization In situ hybridization Somatolactin expression Pituitary Carp Fish LÓPEZ,MAURICIO FIGUEROA,JAIME KAUSEL,GUDRUN VERA,MARÍA INÉS KRAUSKOPF,MANUEL In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
description	We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the regulation of the somatolactin detected with the probe used at the transcriptional level in pituitary glands
author	LÓPEZ,MAURICIO FIGUEROA,JAIME KAUSEL,GUDRUN VERA,MARÍA INÉS KRAUSKOPF,MANUEL
author_facet	LÓPEZ,MAURICIO FIGUEROA,JAIME KAUSEL,GUDRUN VERA,MARÍA INÉS KRAUSKOPF,MANUEL
author_sort	LÓPEZ,MAURICIO
title	In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
title_short	In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
title_full	In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
title_fullStr	In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
title_full_unstemmed	In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
title_sort	in situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (cyprinus carpio)
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010
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In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)

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