A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct

Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	RIOS,MARIANA, OJEDA,SUANY, VELASQUEZ,LUIS A, MAISEY,KEVIN, CROXATTO,HORACIO B
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	differential display estradiol gene mRNA oviduct
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602001000100011
record_format	dspace
spelling	oai:scielo:S0716-976020010001000112001-09-27A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviductRIOS,MARIANAOJEDA,SUANYVELASQUEZ,LUIS AMAISEY,KEVINCROXATTO,HORACIO B differential display estradiol gene mRNA oviduct Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and sequenced. A ribonuclease protection assay confirmed that clone 25 is up-regulated by E2 in the oviduct at 30, 180 and 360 min. This clone exhibited no homology with known genes and in situ hybridization showed it is only expressed in the epithelial cells of the isthmic segment. Clone 25 is likely to represent a new gene, which is up-regulated by E2 in the epithelium of the isthmic segment of the rat oviduct. Its time frame of response is compatible with a mediator of the effect of E2 on oviductal embryo transportinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.1 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011en10.4067/S0716-97602001000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	differential display estradiol gene mRNA oviduct
spellingShingle	differential display estradiol gene mRNA oviduct RIOS,MARIANA OJEDA,SUANY VELASQUEZ,LUIS A MAISEY,KEVIN CROXATTO,HORACIO B A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
description	Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and sequenced. A ribonuclease protection assay confirmed that clone 25 is up-regulated by E2 in the oviduct at 30, 180 and 360 min. This clone exhibited no homology with known genes and in situ hybridization showed it is only expressed in the epithelial cells of the isthmic segment. Clone 25 is likely to represent a new gene, which is up-regulated by E2 in the epithelium of the isthmic segment of the rat oviduct. Its time frame of response is compatible with a mediator of the effect of E2 on oviductal embryo transport
author	RIOS,MARIANA OJEDA,SUANY VELASQUEZ,LUIS A MAISEY,KEVIN CROXATTO,HORACIO B
author_facet	RIOS,MARIANA OJEDA,SUANY VELASQUEZ,LUIS A MAISEY,KEVIN CROXATTO,HORACIO B
author_sort	RIOS,MARIANA
title	A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
title_short	A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
title_full	A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
title_fullStr	A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
title_full_unstemmed	A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
title_sort	segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011
work_keys_str_mv	AT riosmariana asegmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT ojedasuany asegmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT velasquezluisa asegmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT maiseykevin asegmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT croxattohoraciob asegmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT riosmariana segmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT ojedasuany segmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT velasquezluisa segmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT maiseykevin segmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct AT croxattohoraciob segmentandepitheliumspecificmessengerribonucleicacidfragmentupregulatedbyestradiolintheratoviduct
_version_	1718441324921749504

A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct

Ejemplares similares