Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata

Lemon seedlings inoculated with Alternaria alternata develop a hypersensitive response (HR) that includes the induction of Phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone. The signal transduction pathway involved in the development of this response is unknown. We used...

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Autores principales:	ORTEGA,XIMENA, PÉREZ,LUZ M.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	Citrus limon IP3 phosphoinositides Rutaceae signal transduction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100014
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spelling	oai:scielo:S0716-976020010001000142001-09-27Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternataORTEGA,XIMENAPÉREZ,LUZ M. Citrus limon IP3 phosphoinositides Rutaceae signal transduction Lemon seedlings inoculated with Alternaria alternata develop a hypersensitive response (HR) that includes the induction of Phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone. The signal transduction pathway involved in the development of this response is unknown. We used several inhibitors of the Phosphoinositide (PI) animal system to study a possible role of Inositol-1,4,5-triphosphate (IP3 ) in the transduction of the fungal conidia signal in Citrus limon. The HR was only partially inhibited by EGTA, suggesting that not only external but internal calcium as well are necessary for a complete development of the HR. In this plant system, Alternaria alternata induced an early accumulation of the second messenger IP3. When lemon seedlings were watered long term with LiCl, an inhibitor of the phosphoinositide cycle, the IP3 production was reduced, and the LiCl-watered plants could neither induce PAL nor synthesize scoparone in response to fungal conidia. Furthermore, neomycin, a Phospholipase C (PLC, E. C. 3.1.4.3) inhibitor, also inhibited PAL induction and scoparone synthesis in response to A. alternata. These results suggest that IP3 could be involved in the signal transduction pathway for the development of the HR of Citrus limon against A. alternatainfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.1 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100014en10.4067/S0716-97602001000100014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Citrus limon IP3 phosphoinositides Rutaceae signal transduction
spellingShingle	Citrus limon IP3 phosphoinositides Rutaceae signal transduction ORTEGA,XIMENA PÉREZ,LUZ M. Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
description	Lemon seedlings inoculated with Alternaria alternata develop a hypersensitive response (HR) that includes the induction of Phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone. The signal transduction pathway involved in the development of this response is unknown. We used several inhibitors of the Phosphoinositide (PI) animal system to study a possible role of Inositol-1,4,5-triphosphate (IP3 ) in the transduction of the fungal conidia signal in Citrus limon. The HR was only partially inhibited by EGTA, suggesting that not only external but internal calcium as well are necessary for a complete development of the HR. In this plant system, Alternaria alternata induced an early accumulation of the second messenger IP3. When lemon seedlings were watered long term with LiCl, an inhibitor of the phosphoinositide cycle, the IP3 production was reduced, and the LiCl-watered plants could neither induce PAL nor synthesize scoparone in response to fungal conidia. Furthermore, neomycin, a Phospholipase C (PLC, E. C. 3.1.4.3) inhibitor, also inhibited PAL induction and scoparone synthesis in response to A. alternata. These results suggest that IP3 could be involved in the signal transduction pathway for the development of the HR of Citrus limon against A. alternata
author	ORTEGA,XIMENA PÉREZ,LUZ M.
author_facet	ORTEGA,XIMENA PÉREZ,LUZ M.
author_sort	ORTEGA,XIMENA
title	Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
title_short	Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
title_full	Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
title_fullStr	Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
title_full_unstemmed	Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
title_sort	participation of the phosphoinositide metabolism in the hypersensitive response of citrus limon against alternaria alternata
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100014
work_keys_str_mv	AT ortegaximena participationofthephosphoinositidemetabolisminthehypersensitiveresponseofcitruslimonagainstalternariaalternata AT perezluzm participationofthephosphoinositidemetabolisminthehypersensitiveresponseofcitruslimonagainstalternariaalternata
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Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata

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