Identification of Adenovirus 7h Heterogeneity in the E3 Region

Adenovirus genotype 7h was previously reported to be originated from a recombination event between adenovirus genotypes 7p and 3p. Based on those findings, further characterization of other adenovirus 7h strains become important to determine whether all adenovirus 7h strains arose from a single reco...

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Autores principales:	BRUZZONE,MARISOL, BARRO,MARIO, SPENCER,EUGENIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	adenovirus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200010
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spelling	oai:scielo:S0716-976020010002000102002-01-02Identification of Adenovirus 7h Heterogeneity in the E3 RegionBRUZZONE,MARISOLBARRO,MARIOSPENCER,EUGENIO adenovirus Adenovirus genotype 7h was previously reported to be originated from a recombination event between adenovirus genotypes 7p and 3p. Based on those findings, further characterization of other adenovirus 7h strains become important to determine whether all adenovirus 7h strains arose from a single recombinational event. To explore such a possibility, 160 clinical isolates were studied after developing a PCR assay using a primer set designed to amplify the region corresponding to E3-7,7 Kd of adenovirus ADV 7p and E3-9 Kd of adenovirus 3p. The assay was able to differentiate most of the subgenus B strains from adeno 7h with the genotype 3d. The study of several adenovirus 7h clinical isolates revealed the existence of three variants of adeno7h. One of the variants, 7h3, shows a high degree of similarity with gene E3-9 Kd of ADV 3p, but lacks the corresponding AUG codon. Our results suggest that more than one recombination event may explain the detection of three different types of adenovirus 7h. The genotype variants of adeno 7h were detected in different years, indicating that the recombination events took place independently from each other. The study of the recombination region may allow further understanding of the function of several viral polypeptides in the immune response, and understanding the mechanism involved in virulence associated to adenovirus 7h.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.2 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200010en10.4067/S0716-97602001000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	adenovirus
spellingShingle	adenovirus BRUZZONE,MARISOL BARRO,MARIO SPENCER,EUGENIO Identification of Adenovirus 7h Heterogeneity in the E3 Region
description	Adenovirus genotype 7h was previously reported to be originated from a recombination event between adenovirus genotypes 7p and 3p. Based on those findings, further characterization of other adenovirus 7h strains become important to determine whether all adenovirus 7h strains arose from a single recombinational event. To explore such a possibility, 160 clinical isolates were studied after developing a PCR assay using a primer set designed to amplify the region corresponding to E3-7,7 Kd of adenovirus ADV 7p and E3-9 Kd of adenovirus 3p. The assay was able to differentiate most of the subgenus B strains from adeno 7h with the genotype 3d. The study of several adenovirus 7h clinical isolates revealed the existence of three variants of adeno7h. One of the variants, 7h3, shows a high degree of similarity with gene E3-9 Kd of ADV 3p, but lacks the corresponding AUG codon. Our results suggest that more than one recombination event may explain the detection of three different types of adenovirus 7h. The genotype variants of adeno 7h were detected in different years, indicating that the recombination events took place independently from each other. The study of the recombination region may allow further understanding of the function of several viral polypeptides in the immune response, and understanding the mechanism involved in virulence associated to adenovirus 7h.
author	BRUZZONE,MARISOL BARRO,MARIO SPENCER,EUGENIO
author_facet	BRUZZONE,MARISOL BARRO,MARIO SPENCER,EUGENIO
author_sort	BRUZZONE,MARISOL
title	Identification of Adenovirus 7h Heterogeneity in the E3 Region
title_short	Identification of Adenovirus 7h Heterogeneity in the E3 Region
title_full	Identification of Adenovirus 7h Heterogeneity in the E3 Region
title_fullStr	Identification of Adenovirus 7h Heterogeneity in the E3 Region
title_full_unstemmed	Identification of Adenovirus 7h Heterogeneity in the E3 Region
title_sort	identification of adenovirus 7h heterogeneity in the e3 region
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200010
work_keys_str_mv	AT bruzzonemarisol identificationofadenovirus7hheterogeneityinthee3region AT barromario identificationofadenovirus7hheterogeneityinthee3region AT spencereugenio identificationofadenovirus7hheterogeneityinthee3region
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Identification of Adenovirus 7h Heterogeneity in the E3 Region

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