Targeting and fusion proteins during mammalian spermiogenesis

Regulated exocytosis is controlled by internal and external signals. The molecular machinery controlling the sorting from the newly synthesized vesicles from the Golgi apparatus to the plasma membrane play a key role in the regulation of both the number and spatial location of the vesicles. In this...

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Autores principales:	RAMALHO-SANTOS,JOÃO, MORENO,RICARDO D.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	acrosome spermatid Golgi fertilization spermiogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200021
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spelling	oai:scielo:S0716-976020010002000212005-07-11Targeting and fusion proteins during mammalian spermiogenesisRAMALHO-SANTOS,JOÃOMORENO,RICARDO D. acrosome spermatid Golgi fertilization spermiogenesis Regulated exocytosis is controlled by internal and external signals. The molecular machinery controlling the sorting from the newly synthesized vesicles from the Golgi apparatus to the plasma membrane play a key role in the regulation of both the number and spatial location of the vesicles. In this context the mammalian acrosome is a unique vesicle since it is the only secretory vesicle attached to the nucleus. In this work we have studied the membrane trafficking between the Golgi apparatus and the acrosome during mammalian spermiogenesis. During bovine spermiogenesis, Golgi antigens (mannosidase II) were detected in the acrosome until the late cap-phase spermatids, but are not found in testicular spermatozoa (maturation-phase spermatids). This suggests that Golgi-acrosome flow may be relatively unselective, with Golgi residents retrieved before spermiation is complete. Surprisingly, rab7, a protein involved in lysosome/endosome trafficking was also found associated with the acrosomal vesicle during mouse spermiogenesis. Our results suggest that the acrosome biogenesis is associated with membrane flow from both the Golgi apparatus and the endosome/lysosome system in mammalian spermatids.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.2 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200021en10.4067/S0716-97602001000200021
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	acrosome spermatid Golgi fertilization spermiogenesis
spellingShingle	acrosome spermatid Golgi fertilization spermiogenesis RAMALHO-SANTOS,JOÃO MORENO,RICARDO D. Targeting and fusion proteins during mammalian spermiogenesis
description	Regulated exocytosis is controlled by internal and external signals. The molecular machinery controlling the sorting from the newly synthesized vesicles from the Golgi apparatus to the plasma membrane play a key role in the regulation of both the number and spatial location of the vesicles. In this context the mammalian acrosome is a unique vesicle since it is the only secretory vesicle attached to the nucleus. In this work we have studied the membrane trafficking between the Golgi apparatus and the acrosome during mammalian spermiogenesis. During bovine spermiogenesis, Golgi antigens (mannosidase II) were detected in the acrosome until the late cap-phase spermatids, but are not found in testicular spermatozoa (maturation-phase spermatids). This suggests that Golgi-acrosome flow may be relatively unselective, with Golgi residents retrieved before spermiation is complete. Surprisingly, rab7, a protein involved in lysosome/endosome trafficking was also found associated with the acrosomal vesicle during mouse spermiogenesis. Our results suggest that the acrosome biogenesis is associated with membrane flow from both the Golgi apparatus and the endosome/lysosome system in mammalian spermatids.
author	RAMALHO-SANTOS,JOÃO MORENO,RICARDO D.
author_facet	RAMALHO-SANTOS,JOÃO MORENO,RICARDO D.
author_sort	RAMALHO-SANTOS,JOÃO
title	Targeting and fusion proteins during mammalian spermiogenesis
title_short	Targeting and fusion proteins during mammalian spermiogenesis
title_full	Targeting and fusion proteins during mammalian spermiogenesis
title_fullStr	Targeting and fusion proteins during mammalian spermiogenesis
title_full_unstemmed	Targeting and fusion proteins during mammalian spermiogenesis
title_sort	targeting and fusion proteins during mammalian spermiogenesis
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200021
work_keys_str_mv	AT ramalhosantosjoao targetingandfusionproteinsduringmammalianspermiogenesis AT morenoricardod targetingandfusionproteinsduringmammalianspermiogenesis
_version_	1718441331600130048

Targeting and fusion proteins during mammalian spermiogenesis

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