Transcriptome analysis and crop improvement: (A review)
The identification and characterization of differential gene expression from tissues subjected to stress has gained much attention in plant research. The recognition of elements involved in the response to a particular stress enhances the possibility of promoting crop improvement through direct gene...
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| Lenguaje: | English |
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Sociedad de Biología de Chile
2001
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oai:scielo:S0716-976020010003000032002-06-25Transcriptome analysis and crop improvement: (A review)DUNWELL,JIM MMOYA-LEÓN,MARIA AHERRERA,RAULThe identification and characterization of differential gene expression from tissues subjected to stress has gained much attention in plant research. The recognition of elements involved in the response to a particular stress enhances the possibility of promoting crop improvement through direct genetic modification. However, the performance of some of the `first generation' of transgenic plants with the incorporation of a single gene has not always been as expected. These results have stimulated the development of new transgenic constructions introducing more than one gene and capable of modifying complex pathways. Several techniques are available to conduct the analysis of gene regulation, with such information providing the basis for novel constructs specifically designed to modify metabolism. This review deals with techniques that allow the identification and characterization of differentially-expressed genes and the use of molecular pathway information to produce transgenic plants. (Biol Res 2001; 34 3-4: 153-164)info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.3-4 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300003en10.4067/S0716-97602001000300003 |
| institution |
Scielo Chile |
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Scielo Chile |
| language |
English |
| description |
The identification and characterization of differential gene expression from tissues subjected to stress has gained much attention in plant research. The recognition of elements involved in the response to a particular stress enhances the possibility of promoting crop improvement through direct genetic modification. However, the performance of some of the `first generation' of transgenic plants with the incorporation of a single gene has not always been as expected. These results have stimulated the development of new transgenic constructions introducing more than one gene and capable of modifying complex pathways. Several techniques are available to conduct the analysis of gene regulation, with such information providing the basis for novel constructs specifically designed to modify metabolism. This review deals with techniques that allow the identification and characterization of differentially-expressed genes and the use of molecular pathway information to produce transgenic plants. (Biol Res 2001; 34 3-4: 153-164) |
| author |
DUNWELL,JIM M MOYA-LEÓN,MARIA A HERRERA,RAUL |
| spellingShingle |
DUNWELL,JIM M MOYA-LEÓN,MARIA A HERRERA,RAUL Transcriptome analysis and crop improvement: (A review) |
| author_facet |
DUNWELL,JIM M MOYA-LEÓN,MARIA A HERRERA,RAUL |
| author_sort |
DUNWELL,JIM M |
| title |
Transcriptome analysis and crop improvement: (A review) |
| title_short |
Transcriptome analysis and crop improvement: (A review) |
| title_full |
Transcriptome analysis and crop improvement: (A review) |
| title_fullStr |
Transcriptome analysis and crop improvement: (A review) |
| title_full_unstemmed |
Transcriptome analysis and crop improvement: (A review) |
| title_sort |
transcriptome analysis and crop improvement: (a review) |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2001 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300003 |
| work_keys_str_mv |
AT dunwelljimm transcriptomeanalysisandcropimprovementareview AT moyaleonmariaa transcriptomeanalysisandcropimprovementareview AT herreraraul transcriptomeanalysisandcropimprovementareview |
| _version_ |
1718441332353007616 |