Structure analysis of the endoxylanase A gene from Penicillium purpurogenum

Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protei...

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Autores principales:	CHÁVEZ,RENATO, ALMARZA,CAROLINA, SCHACHTER,KAREN, PEIRANO,ALESSANDRA, BULL,PAULINA, EYZAGUIRRE,JAIME
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	family 10 endoxylanases gene structure Penicillium purpurogenum
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009
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spelling	oai:scielo:S0716-976020010003000092002-06-25Structure analysis of the endoxylanase A gene from Penicillium purpurogenumCHÁVEZ,RENATOALMARZA,CAROLINASCHACHTER,KARENPEIRANO,ALESSANDRABULL,PAULINAEYZAGUIRRE,JAIME family 10 endoxylanases gene structure Penicillium purpurogenum Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329 residues (including the signal peptide), and the calculated molecular mass of the mature protein is 31,668 Da. Immunodetection assays of the expressed gene positively identified it as xynA, and sequence alignments indicate a high degree of similarity with family 10 endoxylanases. It is concluded that P. purpurogenum produces endoxylanases of family 10 and 11. The complementary action of endoxylanases of both families may be important for an efficient degradation of xylan by the fungus.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.3-4 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009en10.4067/S0716-97602001000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	family 10 endoxylanases gene structure Penicillium purpurogenum
spellingShingle	family 10 endoxylanases gene structure Penicillium purpurogenum CHÁVEZ,RENATO ALMARZA,CAROLINA SCHACHTER,KAREN PEIRANO,ALESSANDRA BULL,PAULINA EYZAGUIRRE,JAIME Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
description	Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329 residues (including the signal peptide), and the calculated molecular mass of the mature protein is 31,668 Da. Immunodetection assays of the expressed gene positively identified it as xynA, and sequence alignments indicate a high degree of similarity with family 10 endoxylanases. It is concluded that P. purpurogenum produces endoxylanases of family 10 and 11. The complementary action of endoxylanases of both families may be important for an efficient degradation of xylan by the fungus.
author	CHÁVEZ,RENATO ALMARZA,CAROLINA SCHACHTER,KAREN PEIRANO,ALESSANDRA BULL,PAULINA EYZAGUIRRE,JAIME
author_facet	CHÁVEZ,RENATO ALMARZA,CAROLINA SCHACHTER,KAREN PEIRANO,ALESSANDRA BULL,PAULINA EYZAGUIRRE,JAIME
author_sort	CHÁVEZ,RENATO
title	Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
title_short	Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
title_full	Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
title_fullStr	Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
title_full_unstemmed	Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
title_sort	structure analysis of the endoxylanase a gene from penicillium purpurogenum
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009
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Structure analysis of the endoxylanase A gene from Penicillium purpurogenum

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