Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant

Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: ß-MAAC). Methylcellulose discs instilled with...

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Autores principales:	LEMUS,DAVID, DABANCENS,ALFREDO, ILLANES,JULIO, FUENZALIDA,MARCELA, GUERRERO,ANIBAL, LÓPEZ,CLAUDIA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2001
Materias:	antiangiogenesis betamethasone CAM tumor
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300010
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spelling	oai:scielo:S0716-976020010003000102002-06-25Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatantLEMUS,DAVIDDABANCENS,ALFREDOILLANES,JULIOFUENZALIDA,MARCELAGUERRERO,ANIBALLÓPEZ,CLAUDIA antiangiogenesis betamethasone CAM tumor Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: ß-MAAC). Methylcellulose discs instilled with 10 µl of ß-MAAC (0.08 µg/ml),10 µl of tumor supernatant(TA3ts),5 µl ß-MAAC + 5 µl TA3ts, and 10 µl of PBS as control were implanted in host chick eggs. On day 12, the grafts were removed, photographed and fixed. Sections were stained in parallel, one and three with hematoxylin-eosin, and section two by the Tunel method. The number of vessels was evaluated in a microscopic field of the CAM (2250 µm2 ). The results show that ß-MAAC produced a significant inhibition of neovascularization in comparison to that observed in controls (P < 0.0025; Student t-Test). Discs instilled with TA3ts produced an intense stimulation of angiogenesis in contrast, when discs were instilled with 5 µl of ß-MAAC + 5 µl of TA3ts the angiogenesis was significantly inhibited (P< 0.001). The results show that effective antiangiogenic doses of betamethasone are in the range of 10-7 M, (probably a genomic mediated action) and that this effect of low concentration may have clinical applications.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.34 n.3-4 20012001-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300010en10.4067/S0716-97602001000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antiangiogenesis betamethasone CAM tumor
spellingShingle	antiangiogenesis betamethasone CAM tumor LEMUS,DAVID DABANCENS,ALFREDO ILLANES,JULIO FUENZALIDA,MARCELA GUERRERO,ANIBAL LÓPEZ,CLAUDIA Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
description	Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: ß-MAAC). Methylcellulose discs instilled with 10 µl of ß-MAAC (0.08 µg/ml),10 µl of tumor supernatant(TA3ts),5 µl ß-MAAC + 5 µl TA3ts, and 10 µl of PBS as control were implanted in host chick eggs. On day 12, the grafts were removed, photographed and fixed. Sections were stained in parallel, one and three with hematoxylin-eosin, and section two by the Tunel method. The number of vessels was evaluated in a microscopic field of the CAM (2250 µm2 ). The results show that ß-MAAC produced a significant inhibition of neovascularization in comparison to that observed in controls (P < 0.0025; Student t-Test). Discs instilled with TA3ts produced an intense stimulation of angiogenesis in contrast, when discs were instilled with 5 µl of ß-MAAC + 5 µl of TA3ts the angiogenesis was significantly inhibited (P< 0.001). The results show that effective antiangiogenic doses of betamethasone are in the range of 10-7 M, (probably a genomic mediated action) and that this effect of low concentration may have clinical applications.
author	LEMUS,DAVID DABANCENS,ALFREDO ILLANES,JULIO FUENZALIDA,MARCELA GUERRERO,ANIBAL LÓPEZ,CLAUDIA
author_facet	LEMUS,DAVID DABANCENS,ALFREDO ILLANES,JULIO FUENZALIDA,MARCELA GUERRERO,ANIBAL LÓPEZ,CLAUDIA
author_sort	LEMUS,DAVID
title	Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
title_short	Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
title_full	Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
title_fullStr	Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
title_full_unstemmed	Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
title_sort	antiangiogenic effect of betamethasone on the chick cam stimulated by ta3 tumor supernatant
publisher	Sociedad de Biología de Chile
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300010
work_keys_str_mv	AT lemusdavid antiangiogeniceffectofbetamethasoneonthechickcamstimulatedbyta3tumorsupernatant AT dabancensalfredo antiangiogeniceffectofbetamethasoneonthechickcamstimulatedbyta3tumorsupernatant AT illanesjulio antiangiogeniceffectofbetamethasoneonthechickcamstimulatedbyta3tumorsupernatant AT fuenzalidamarcela antiangiogeniceffectofbetamethasoneonthechickcamstimulatedbyta3tumorsupernatant AT guerreroanibal antiangiogeniceffectofbetamethasoneonthechickcamstimulatedbyta3tumorsupernatant AT lopezclaudia antiangiogeniceffectofbetamethasoneonthechickcamstimulatedbyta3tumorsupernatant
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Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant

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