Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans

Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G...

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Autores principales:	CHIUCHETTA,SIMONE JUREMA RUGGERI, CASTRO-PRADO,MARIALBA AVEZUM ALVES DE
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	aneuploidy antineoplastic drug Aspergillus nidulans recombinagenic activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006
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spelling	oai:scielo:S0716-976020020001000062003-01-28Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulansCHIUCHETTA,SIMONE JUREMA RUGGERICASTRO-PRADO,MARIALBA AVEZUM ALVES DE aneuploidy antineoplastic drug Aspergillus nidulans recombinagenic activity Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G2 phase, they provide an excellent system for the study of mitotic crossing-over. Taking into account that mitotic crossing-over occurs during G2 period, the evaluation of recombinagenic and aneugenic potential of vincristine is provided with regard to two diploid strains of A. nidulans: a wild strain (uvsH+//uvsH+) and a defective one in DNA repair (uvsH//uvsH). Drug toxicity and its effect on the asexual cycle of A. nidulans has been evaluated as well. Treatment of both strains with vincristine did not change colony growth in the culture, however cytological analyses showed aberrant conidiophores. Recombinagenic potential of vincristine was evaluated by induction of gene homozygosis originally present in heterozygosity diploid strains (Homozygotization Index). Results show that vincristine induces mitotic crossing-over and higher frequency of aneuploid mitotic segregants. The results also show the recombinagenic and aneuploidogenic potential of vincristine and suggest its participation in the induction of secondary malignanciesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.1 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006en10.4067/S0716-97602002000100006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	aneuploidy antineoplastic drug Aspergillus nidulans recombinagenic activity
spellingShingle	aneuploidy antineoplastic drug Aspergillus nidulans recombinagenic activity CHIUCHETTA,SIMONE JUREMA RUGGERI CASTRO-PRADO,MARIALBA AVEZUM ALVES DE Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
description	Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G2 phase, they provide an excellent system for the study of mitotic crossing-over. Taking into account that mitotic crossing-over occurs during G2 period, the evaluation of recombinagenic and aneugenic potential of vincristine is provided with regard to two diploid strains of A. nidulans: a wild strain (uvsH+//uvsH+) and a defective one in DNA repair (uvsH//uvsH). Drug toxicity and its effect on the asexual cycle of A. nidulans has been evaluated as well. Treatment of both strains with vincristine did not change colony growth in the culture, however cytological analyses showed aberrant conidiophores. Recombinagenic potential of vincristine was evaluated by induction of gene homozygosis originally present in heterozygosity diploid strains (Homozygotization Index). Results show that vincristine induces mitotic crossing-over and higher frequency of aneuploid mitotic segregants. The results also show the recombinagenic and aneuploidogenic potential of vincristine and suggest its participation in the induction of secondary malignancies
author	CHIUCHETTA,SIMONE JUREMA RUGGERI CASTRO-PRADO,MARIALBA AVEZUM ALVES DE
author_facet	CHIUCHETTA,SIMONE JUREMA RUGGERI CASTRO-PRADO,MARIALBA AVEZUM ALVES DE
author_sort	CHIUCHETTA,SIMONE JUREMA RUGGERI
title	Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
title_short	Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
title_full	Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
title_fullStr	Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
title_full_unstemmed	Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
title_sort	vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of aspergillus nidulans
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006
work_keys_str_mv	AT chiuchettasimonejuremaruggeri vincristineinducessomaticsegregationviamitoticcrossingoverindiploidcellsofaspergillusnidulans AT castropradomarialbaavezumalvesde vincristineinducessomaticsegregationviamitoticcrossingoverindiploidcellsofaspergillusnidulans
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Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans

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